Production of sophorolipids from winterization oil cake by solid-state fermentation : optimization, monitoring and effect of mixing
Jiménez Peñalver, Pedro (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Gea Leiva, Teresa (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Sanchez, Antoni (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Font i Segura, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Date:	2016
Abstract:	Sophorolipids (SLs) are a group of extracellular biosurfactants produced by the yeast Starmerella bombicola. The present study explored the use of winterization oil cake (WOC), a residual oil cake that comes from the oil refining industry, as a substrate for the production of SLs by solid-state fermentation (SSF). Sugar beet molasses (MOL) was used as a co-substrate and S. bombicola ATCC 22214 as the inoculum. Fermentation was performed on the 100-g scale and was optimized in terms of the ratio of substrates and the aeration rate using response surface methodology. The optimized SSF process (1:4 MOL:WOC mass ratio and 0. 30 L kg⁻¹ min⁻¹ aeration rate), carried out under static conditions, was monitored for 10 days with a maximum SL yield of 0. 179 g per g DM (dry matter). The effect of intermittent mixing on the process was also investigated. Mixing caused a 31% increase in SL production, with a total yield of 0. 235 g per g DM. The Oxygen Uptake Rate (OUR) and the Cumulative Oxygen Consumption (COC) were used to monitor the biological activity of the fermentation processes. There were significant correlations between the SL yield and the oxygen and fats consumed. The SLs were characterized by FTIR and ¹H NMR analysis.
Grants:	Ministerio de Economía y Competitividad CTM2015-69513-R Ministerio de Economía y Competitividad BES-2013-064852
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Published in:	Biochemical engineering journal, Vol. 115 (Nov. 2016) , p. 93-100, ISSN 1369-703X

DOI: 10.1016/j.bej.2016.08.006

Preprint 31 p, 857.5 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Engineering > Composting Research Group (GICOM)
Articles > Research articles
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