Long term enhanced solid-state fermentation : inoculation strategies for amylase production from soy and bread wastes by Thermomyces sp. in a sequential batch operation
Cerda Llanos, Alejandra Patricia (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
El-Bakry, Mamdouh (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Gea Leiva, Teresa (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Sanchez, Antoni (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Date:	2016
Abstract:	The effect of two thermophilic amylase producing strains was evaluated using different mixtures of soy and bread wastes. Thermomyces sp. was found to be better inoculum than Geobacillus sp. for a soy and bread waste mixture (90:10 w/w respectively) producing a maximum enzyme activity of 39. 9·103 U g−1 dry substrate. Three strategies (a, b, c) were evaluated for solid-state fermentation (SSF) operation in sequential batches. Fermented solids from each batch were used to inoculate the following batch: (a) solids at the moment of maximum biological activity; (b) final solids (end of the process, maximum amylase production); (c) final solids after enzymatic extraction. The evaluated strategies led to an increase in amylase production of 50, 500 and 98% for each strategy, respectively. This indicates the suitability of fermented solids to act as inoculum and the enhancement of amylase production compared to traditional batches. As one of the main challenges of SSF is the maintenance of a productive process along time, these results confirm SSF as an excellent option to produce amylases from organic wastes.
Grants:	Ministerio de Economía y Competitividad CTM2015-69513-R
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Subject:	Solid-state fermentation ; Thermomyces ; Inoculation strategies ; Amylaseamylase ; Sequential batch ; Waste valorisation
Published in:	Journal of environmental chemical engineering, Vol. 4, issue 2 (June 2016) , p. 2394-2401, ISSN 2213-3437

DOI: 10.1016/j.jece.2016.04.022

Preprint 29 p, 568.2 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Engineering > Composting Research Group (GICOM)
Articles > Research articles
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