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Página principal > Artículos > Artículos publicados > Annexin-V/quantum dot probes for multimodal apoptosis monitoring in living cells : |
Fecha: | 2015 |
Resumen: | There is a great demand to develop novel techniques that allow useful and complete monitoring of apoptosis, which is a key factor of several diseases and a target for drug development. Here, we present the use of a novel dual electrochemical/optical label for the detection and study of apoptosis. We combined the specificity of Annexin-V for phosphatidylserine, a phospholipid expressed in the outer membrane of apoptotic cells, with the optical and electrochemical properties of quantum dots to create a more efficient label. Using this conjugate we addressed three important issues: (i) we made the labeling of apoptotic cells faster (30 min) and easier; (ii) we fully characterized the samples by common cell biological techniques (confocal laser scanning microscopy, scanning electron microscopy and flow cytometry); and (III) we developed a fast, cheap and quantitative electrochemical detection method for apoptotic cells with results in full agreement with those obtained by flow cytometry. |
Ayudas: | European Commission 613844 Ministerio de Ciencia e Innovación MAT2011-25870 Ministerio de Ciencia e Innovación TEC2011-29140-C03-03 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-524 |
Derechos: | Tots els drets reservats |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió acceptada per publicar |
Materia: | Apoptotic cells ; Biological techniques ; Confocal laser scanning microscopy ; Drug development ; Electrochemical detection method ; Novel techniques ; Outer membrane ; Phosphatidylserine ; Annexin A5 ; Apoptosis ; Cell Line ; Dimethyl Sulfoxide ; Electrochemical Techniques ; Flow Cytometry ; Humans ; Microscopy, Confocal ; Microscopy, Electron, Scanning ; Phosphatidylserines ; Protein Binding ; Quantum Dots ; Surface Properties |
Publicado en: | Nanoscale, Vol. 7, Núm. 9 (March 2015) , p. 4097-4104, ISSN 2040-3372 |
Postprint 12 p, 3.7 MB |