Type III secretion inhibitors for the management of bacterial plant diseases
Puigvert, Marina (Centre de Recerca en Agrigenòmica)
Solé Castellví, Montserrat (Centre de Recerca en Agrigenòmica)
López-Garcia, Belén (Centre de Recerca en Agrigenòmica)
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)
Beattie, Karren D. (Griffith Institute for Drug Discovery)
Davis, Rohan (Griffith Institute for Drug Discovery)
Elofsson, Mikael (Umeå University. Department of Chemistry)
Valls, Marc (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Fecha:	2019
Resumen:	The identification of chemical compounds that prevent and combat bacterial diseases is fundamental for crop production. Bacterial virulence inhibitors are a promising alternative to classical control treatments, because they have a low environmental impact and are less likely to generate bacterial resistance. The major virulence determinant of most animal and plant bacterial pathogens is the type III secretion system (T3SS). In this work, we screened nine plant extracts and 12 isolated compounds-including molecules effective against human pathogens-for their capacity to inhibit the T3SS of plant pathogens and for their applicability as virulence inhibitors for crop protection. The screen was performed using a luminescent reporter system developed in the model pathogenic bacterium Ralstonia solanacearum. Five synthetic molecules, one natural product and two plant extracts were found to down-regulate T3SS transcription, most through the inhibition of the regulator hrpB. In addition, for three of the molecules, corresponding to salicylidene acylhydrazide derivatives, the inhibitory effect caused a dramatic decrease in the secretion capacity, which was translated into impaired plant responses. These candidate virulence inhibitors were then tested for their ability to protect plants. We demonstrated that salicylidene acylhydrazides can limit R. solanacearum multiplication in planta and protect tomato plants from bacterial speck caused by Pseudomonas syringae pv. tomato. Our work validates the efficiency of transcription reporters to discover compounds or natural product extracts that can be potentially applied to prevent bacterial plant diseases.
Ayudas:	Ministerio de Economía y Competitividad AGL2016-78002-R Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad RyC2014-16158 Ministerio de Economía y Competitividad AGL2013-46898-R
Nota:	Altres ajuts: COST Action SUSTAIN (FA1208) from the European Union.
Derechos:	Tots els drets reservats.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Bacterial speck ; Bacterial wilt ; Disease control ; Pseudomonas syringae ; Ralstonia solanacearum ; Type III secretion system ; Virulence inhibitors
Publicado en:	Molecular plant pathology, Vol. 20, issue 1 (Jan. 2019) , p. 20-32, ISSN 1364-3703

DOI: 10.1111/mpp.12736
PMID: 30062690

Postprint 36 p, 1.9 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > CRAG (Centro de Investigación en Agrigenómica)
Artículos > Artículos de investigación
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