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Expression dynamics of innate immunity in influenza virus-infected swine
Montoya, Maria (Universitat Autònoma de Barcelona. Centre de Recerca en Sanitat Animal)
Foni, Emanuela (OIE Reference Laboratory for Swine Influenza. Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna)
Solórzano, Alicia (The State University of New Jersey. Public Health Research Institute and Regional Biocontainment Laboratory)
Razzuoli, Elisabetta (S.S. Sezione Genova. Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta)
Baratelli, Massimiliano (Universitat Autònoma de Barcelona. Centre de Recerca en Sanitat Animal)
Bilato, Dania (Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna. Laboratory of Cellular Immunology)
Córdoba, Lorena (Universitat Autònoma de Barcelona. Centre de Recerca en Sanitat Animal)
del Burgo, Maria Angeles Martín (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Departmento de Biotecnología)
Martinez, Jorge (Universitat Autònoma de Barcelona. Departament de Sanitat i Anatomia Animals)
Martinez-Orellana, Pamela (Universitat Autònoma de Barcelona. Centre de Recerca en Sanitat Animal)
Chiapponi, Chiara (Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna. OIE Reference Laboratory for Swine Influenza)
Perlin, David S. (The State University of New Jersey. Public Health Research Institute and Regional Biocontainment Laboratory)
del Real, Gustavo (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Departmento de Biotecnología)
Amadori, Massimo (Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna. Laboratory of Cellular Immunology)

Fecha: 2017
Resumen: The current circulating swine influenza virus (IV) subtypes in Europe (H1N1, H1N2, and H3N2) are associated with clinical outbreaks of disease. However, we showed that pigs could be susceptible to other IV strains that are able to cross the species barrier. In this work, we extended our investigations into whether different IV strains able to cross the species barrier might give rise to different innate immune responses that could be associated with pathological lesions. For this purpose, we used the same samples collected in a previous study of ours, in which healthy pigs had been infected with a H3N2 Swine IV and four different H3N8 IV strains circulating in different animal species. Pigs had been clinically inspected and four subjects/group were sacrificed at 3, 6, and 21 days post infection. In the present study, all groups but mock exhibited antibody responses to IV nucleoprotein protein. Pulmonary lesions and high-titered viral replication were observed in pigs infected with the swine-adapted virus. Interestingly, pigs infected with avian and seal H3N8 strains also showed moderate lesions and viral replication, whereas equine and canine IVs did not cause overt pathological signs, and replication was barely detectable. Swine IV infection induced interferon (IFN)-alpha and interleukin-6 responses in bronchoalveolar fluids (BALF) at day 3 post infection, as opposed to the other non-swine-adapted virus strains. However, IFN-alpha responses to the swine-adapted virus were not associated with an increase of the local, constitutive expression of IFN-alpha genes. Remarkably, the Equine strain gave rise to a Serum Amyloid A response in BALF despite little if any replication. Each virus strain could be associated with expression of cytokine genes and/or proteins after infection. These responses were observed well beyond the period of virus replication, suggesting a prolonged homeostatic imbalance of the innate immune system.
Nota: We would like to thank Dr. Jaime Maldonado and HIPRA, Spain for the A/swine/Spain/54008/2004 (H3N2) influenza virus; Dr. Dubovi and Cornell University for the A/Canine/NY/105447/08 (H3N8) influenza virus; Dr. Chambers and University of Kentucky for the A/Equine/OH/1/03 (H3N8) influenza virus; and Dr. Hon Ip and the US Geological Survey National Wildlife Health Center for the A/American black duck/Maine/44411-532/2008 (H3N8) and the A/Harbour Seal/New Hampshire/179629/2011 (H3N8) influenza viruses. The authors thank Sergio López, David Solanes and Francisco X. Abad for their help during the experimental infections as well as the personnel in Cat3 laboratories and animal house. The authors also wish to thank Dr. I. L. Archetti (IZSLER, Brescia, Italy) for the invaluable help in measuring some clinical immunology parameters, Dr. L. Fraile (UdL, Spain) for assistance in statistical analysis, Dr. J. Domínguez (INIA, Spain) for porcine antibodies, Dr. M. Gennari and Dr. M. Giunta (S.S. Genova, IZSPLV, Italy) for assistance in real-time PCR analyses. The skillful technical assistance of Mrs. C. Mantovani (IZSLER, Brescia, Italy) is also gratefully acknowledged. The research leading to these results has received funding from: the European Community's Seventh Framework Programme (FP7, 2007-2013), Research Infrastructures action, under the grant agreement No. FP7-228393 (NADIR project), and from the project AGL2010-22200-C02-01 of Spanish Ministry of Science and Innovation.
Nota: Número d'acord de subvenció US Geological Survey National Wildlife Health Center/[u'duck/Maine/44411-532/2008', u'H3N8']
Nota: Número d'acord de subvenció Seventh Framework Programme/[u'FP7-228393', u'AGL2010-22200-C02-01']
Derechos: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Lengua: Anglès.
Documento: article ; recerca ; publishedVersion
Materia: Pig ; Influenza ; Virus ; Innate immunity ; Cytokines
Publicado en: Frontiers in Veterinary Science, Vol. 4, Núm. 48 (21 April 2017) , ISSN 2297-1769

DOI: 10.3389/fvets.2017.00048
PMID: 28484702


13 p, 3.2 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
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 Registro creado el 2019-04-01, última modificación el 2019-05-21



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