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Surface modification of microparticles causes differential uptake responses in normal and tumoral human breast epithelial cells
Patiño Padial, Tania (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia)
Soriano Martín, Jorge (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia)
Barrios, L. (Leonardo) (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia)
Ibáñez, Elena (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia)
Nogués, C. (Carme) (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia)

Date: 2015
Abstract: The use of micro- and nanodevices as multifunctional systems for biomedical applications has experienced an exponential growth during the past decades. Although a large number of studies have focused on the design and fabrication of new micro- and nanosystems capable of developing multiple functions, a deeper understanding of their interaction with cells is required. In the present study, we evaluated the effect of different microparticle surfaces on their interaction with normal and tumoral human breast epithelial cell lines. For this, AlexaFluor488 IgG functionalized polystyrene microparticles (3 μm) were coated with Polyethyleneimine (PEI) at two different molecular weights, 25 and 750 kDa. The effect of microparticle surface properties on cytotoxicity, cellular uptake and endocytic pathways were assessed for both normal and tumoral cell lines. Results showed a differential response between the two cell lines regarding uptake efficiency and mechanisms of endocytosis, highlighting the potential role of microparticle surface tunning for specific cell targeting.
Note: Altres ajuts: FI-DGR 2013 de la Generalitat de Catalunya
Note: Número d'acord de subvenció MICINN/TEC2011-29140-C03
Note: Número d'acord de subvenció AGAUR/2014/SGR-524
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Antibodies, Neoplasm ; Breast Neoplasms ; Cell Line, Tumor ; Coated Materials, Biocompatible ; Drug Delivery Systems ; Female ; Humans ; Immunoglobulin G ; Nanoparticles ; Polyethyleneimine
Published in: Scientific Reports, Vol. 5 (June 2015) , art. 11371, ISSN 2045-2322

DOI: 10.1038/srep11371
PMID: 26068810


12 p, 1.3 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2019-04-12, last modified 2019-06-11



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