Analysis of alternative splicing events for cancer diagnosis using a multiplexing nanophotonic biosensor
Huertas, César S. (Institut Català de Nanociència i Nanotecnologia)
Domínguez Zotes, Santos (Institut Català de Nanociència i Nanotecnologia)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)

Date:	2017
Abstract:	Personalized medicine is a promising tool not only for prevention, screening and development of more efficient treatment strategies, but also for diminishing the side effects caused by current therapies. Deciphering gene regulation pathways provides a reliable prognostic analysis to elucidate the origin of grave diseases and facilitate the selection of the most adequate treatment for each individual. Alternative splicing of mRNA precursors is one of these gene regulation pathways and enables cells to generate different protein outputs from the same gene depending on their developmental or homeostatic status. Its deregulation is strongly linked to disease onset and progression constituting a relevant and innovative class of biomarker. Herein we report a highly selective and sensitive nanophotonic biosensor based on the direct monitoring of the aberrant alternative splicing of Fas gene. Unlike conventional methods, the nanobiosensor performs a real-time detection of the specific isoforms in the fM-pM range without any cDNA synthesis or PCR amplification requirements. The nanobiosensor has been proven isoform-specific with no crosshybridization, greatly minimizing detection biases. The demonstrated high sensitivity and specificity make our nanobiosensor ideal for examining significant tumor-associated expression shifts of alternatively spliced isoforms for the early and accurate theranostics of cancer.
Grants:	Ministerio de Ciencia e Innovación TEC2012-3428 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-624 Ministerio de Economía y Competitividad SEV-2013-0295
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Alternative Splicing ; Biosensing Techniques ; DNA ; Interferometry ; Nanoparticles ; Neoplasms ; Photons ; Protein Isoforms ; Silicon Compounds
Published in:	Scientific reports, Vol. 7 (January 2017) , art. 41368, ISSN 2045-2322

DOI: 10.1038/srep41368
PMID: 28120920

8 p, 985.7 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles