Polymer-induced swelling of solid-supported lipid membranes
Kreuzer, Martin (Institut Català de Nanociència i Nanotecnologia)
Trapp, Marcus (Helmholtz-Zentrum Berlin für Materialien und Energie GmbH)
Dahint, Reiner (Ruprecht-Karls-Universität Heidelberg)
Steitz, Roland (Helmholtz-Zentrum Berlin für Materialien und Energie GmbH)

Date:	2016
Abstract:	In this paper, we study the interaction of charged polymers with solid-supported 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes by in-situ neutron reflectivity. We observe an enormous swelling of the oligolamellar lipid bilayer stacks after incubation in solutions of poly(allylamine hydrochloride) (PAH) in DO. The positively charged polyelectrolyte molecules interact with the lipid bilayers and induce a drastic increase in their d-spacing by a factor of ~4. Temperature, time, and pH influence the swollen interfacial lipid linings. From our study, we conclude that electrostatic interactions introduced by the adsorbed PAH are the main cause for the drastic swelling of the lipid coatings. The DMPC membrane stacks do not detach from their solid support at T > T. Steric interactions, also introduced by the PAH molecules, are held responsible for the stabilizing effect. We believe that this novel system offers great potential for fundamental studies of biomembrane properties, keeping the membrane's natural fluidity and freedom, decoupled from a solid support at physiological conditions.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Lipid ; Membranes ; Neutron reflectivity ; Polymer
Published in:	Membranes, Vol. 6, Issue 1 (March 2016) , art. 2, ISSN 2077-0375

DOI: 10.3390/membranes6010002
PMID: 26703746

15 p, 1.4 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles