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The oxidative potential of differently charged silver and gold nanoparticles on three human lung epithelial cell types
Schlinkert, Paul (Paris Lodron-University of Salzburg. Department of Molecular Biology)
Casals, Eudald (Institut Català de Nanociència i Nanotecnologia)
Boyles, Matthew S. P. (Paris Lodron-University of Salzburg. Department of Molecular Biology)
Tischler, Ulrike (Paris Lodron-University of Salzburg. Department of Molecular Biology)
Hornig, Eva (Paris Lodron-University of Salzburg. Department of Molecular Biology)
Tran Thi Thanh, Ngoc (Institut Català de Nanotecnologia)
Zhao, Jiayuan (Institute for Work and Health)
Himly, Martin (Paris Lodron-University of Salzburg. Department of Molecular Biology)
Riediker, Michael (Institue for Occupational Medicine)
Oostingh, G.J. (Salzburg University of Applied Sciences)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
Duschl, Albert (Paris Lodron-University of Salzburg. Department of Molecular Biology)

Date: 2015
Abstract: Background: Nanoparticle (NPs) functionalization has been shown to affect their cellular toxicity. To study this, differently functionalized silver (Ag) and gold (Au) NPs were synthesised, characterised and tested using lung epithelial cell systems. Methods: Monodispersed Ag and Au NPs with a size range of 7 to 10 nm were coated with either sodium citrate or chitosan resulting in surface charges from -50 mV to +70 mV. NP-induced cytotoxicity and oxidative stress were determined using A549 cells, BEAS-2B cells and primary lung epithelial cells (NHBE cells). TEER measurements and immunofluorescence staining of tight junctions were performed to test the growth characteristics of the cells. Cytotoxicity was measured by means of the CellTiter-Blue ® and the lactate dehydrogenase assay and cellular and cell-free reactive oxygen species (ROS) production was measured using the DCFH-DA assay. Results: Different growth characteristics were shown in the three cell types used. A549 cells grew into a confluent mono-layer, BEAS-2B cells grew into a multilayer and NHBE cells did not form a confluent layer. A549 cells were least susceptible towards NPs, irrespective of the NP functionalization. Cytotoxicity in BEAS-2B cells increased when exposed to high positive charged (+65-75 mV) Au NPs. The greatest cytotoxicity was observed in NHBE cells, where both Ag and Au NPs with a charge above +40 mV induced cytotoxicity. ROS production was most prominent in A549 cells where Au NPs (+65-75 mV) induced the highest amount of ROS. In addition, cell-free ROS measurements showed a significant increase in ROS production with an increase in chitosan coating. Conclusions: Chitosan functionalization of NPs, with resultant high surface charges plays an important role in NP-toxicity. Au NPs, which have been shown to be inert and often non-cytotoxic, can become toxic upon coating with certain charged molecules. Notably, these effects are dependent on the core material of the particle, the cell type used for testing and the growth characteristics of these cell culture model systems.
Note: Número d'acord de subvenció EC/FP7/264506
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: article ; recerca ; publishedVersion
Subject: Human lung epithelial cells ; Nanoparticles ; Cytotoxicity ; ROS production ; Surface charge
Published in: Journal of nanobiotechnology, Vol. 13 (January 2015) , art. 1, ISSN 1477-3155

DOI: 10.1186/s12951-014-0062-4
PMID: 25592092

18 p, 2.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles

 Record created 2019-06-03, last modified 2020-08-08

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