Web of Science: 8 citas, Scopus: 8 citas, Google Scholar: citas,
Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma
Peláez Gutiérrez, Enelia Cristina (Institut Català de Nanociència i Nanotecnologia)
Estévez, M. Carmen (Institut Català de Nanociència i Nanotecnologia)
Portela Otaño, Alejandro (Institut Català de Nanociència i Nanotecnologia)
Salvador, J. Pablo (Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN))
Marco, María Pilar (Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN))
Lechuga, Laura (Institut Català de Nanociència i Nanotecnologia)

Fecha: 2018
Resumen: Acenocoumarol (Sintrom®) is an oral anticoagulant prescribed for the treatment of a variety of thromboembolic disorders such as atrial fibrillation and thrombosis or embolism. It inhibits fibrin production preventing clot formation. Acenocoumarol has a narrow therapeutic range, and its effects depend on several factors, such as body weight, age, metabolism, diet, certain medical conditions or the intake of additional drugs, among others. A higher dose may result in the risk of bleeding, while if it is too low, the risk of blood clot can increase. Complementary tools that allow the therapeutic drug monitoring (TDM) of acenocoumarol plasmatic levels from the starting of the treatment would be of paramount importance to personalize the treatment. Point-of-care (POC) devices can offer an added value in facilitating on-site monitoring (i. e. hospitals, primary care doctor or even by the patient itself) and can aid in dosage management. With this aim, we have developed a compact and simple nanoplasmonic sensing device based on gold nanodisks for the rapid monitoring of acenocoumarol, using highly specific polyclonal antibodies produced against this drug. A specific and reproducible label free indirect competitive assay has been developed and the viability of performing the evaluation directly in plasma diluted 1:1 has been demonstrated. A limit of detection (LOD) of only 0. 77 ± 0. 69 nM, an IC of 48. 2 ± 5. 12 nM and a dynamic range between 3. 38 ± 1. 33 nM and 1154 ± 437 nM were achieved, which easily fit within the drug plasma levels of acenocoumarol, making this approach a highly attractive option for its decentralized monitoring in human plasma.
Nota: Número d'acord de subvenció MINECO/SEV-2013-0295
Nota: Número d'acord de subvenció AGAUR/2017/SGR-1441
Derechos: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Lengua: Anglès
Documento: article ; recerca ; acceptedVersion
Materia: Acenocoumarol ; LSPR immunosensor ; Gold nanodisks ; Point-of-care device
Publicado en: Biosensors and bioelectronics, Vol. 119 (November 2018) , p. 149-155, ISSN 1873-4235

DOI: 10.1016/j.bios.2018.08.011


Postprint
16 p, 1.2 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Institut Català de Nanociència i Nanotecnologia (ICN2)
Artículos > Artículos de investigación
Artículos > Artículos publicados

 Registro creado el 2019-07-22, última modificación el 2021-03-03



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