Web of Science: 11 citations, Scopus: 16 citations, Google Scholar: citations,
Inter-laboratory comparison of nanoparticle size measurements using dynamic light scattering and differential centrifugal sedimentation
Langevin, D. (Université Paris Saclay. Centre national de la recherche scientifique. Laboratoire de Physique des Solides)
Lozano, O. (Escuela de Medicina y Ciencias de la Salud. Tecnologico de Monterrey. Cátedra de Cardiología y Medicina Vascular.)
Salvati, A. (University of Groningen. Groningen Research Institute of Pharmacy)
Kestens, V. (European Commission. Joint Research Centre)
Monopoli, M. (University College Dublin. Centre for BioNano Interactions)
Raspaud, E. (Université Paris Saclay. Centre national de la recherche scientifique. Laboratoire de Physique des Solides)
Mariot, S. (Université Paris Saclay. Centre national de la recherche scientifique. Laboratoire de Physique des Solides)
Salonen, A. (Université Paris Saclay. Centre national de la recherche scientifique. Laboratoire de Physique des Solides)
Thomas, S. (University College Dublin. Centre for BioNano Interactions)
Driessen, M. (German Federal Institute for Risk Assessment. Department of Chemical and Product Safety)
Haase, A. (German Federal Institute for Risk Assessment. Department of Chemical and Product Safety)
Nelissen, I. (Flemish Institute for Technological Research. Unit Environmental Risk and Health)
Smisdom, N. (Flemish Institute for Technological Research. Unit Environmental Risk and Health)
Pompa, P.P. (Istituto Italiano di Tecnologia)
Maiorano, G. (Istituto Italiano di Tecnologia)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
Puchowicz, D. (Textile Research Institute. Scientific Department of Unconventional Technologies and Textiles)
Stępnik, M. (Nofer Institute of Occupational Medicine. Department of Toxicology and Carcinogenesis)
Suárez, G. (Institut universitaire romand de Santé au Travail)
Riediker, M. (Nanyang Technological University. School of Materials Science & Engineering)
Benetti, F. (European Center for the Sustainable Impact of Nanotechnology)
Mičetić, I. (European Center for the Sustainable Impact of Nanotechnology)
Venturini, M. (European Center for the Sustainable Impact of Nanotechnology)
Kreyling, W.G. (German Research Center for Environmental Health. Helmholtz Centre Munich)
van der Zande, M. (Wageningen University & Research Centre)
Bouwmeester, H. (Wageningen University & Research Centre)
Milani, S. (Ludwig-Maximilians-University. Soft condensed matter physics)
Rädler, J.O. (Ludwig-Maximilians-University. Soft condensed matter physics)
Mülhopt, S. (Karlsruhe Institute of Technology)
Lynch, I. (University of Birmingham. School of Geography, Earth and Environmental Sciences.)
Dawson, K. (University College Dublin. Centre for BioNano Interactions)

Date: 2018
Abstract: Nanoparticle in vitro toxicity studies often report contradictory results with one main reason being insufficient material characterization. In particular the characterization of nanoparticles in biological media remains challenging. Our aim was to provide robust protocols for two of the most commonly applied techniques for particle sizing, i. e. dynamic light scattering (DLS) and differential centrifugal sedimentation (DCS) that should be readily applicable also for users not specialized in nanoparticle physico-chemical characterization. A large number of participants (40, although not all participated in all rounds) were recruited for a series of inter-laboratory comparison (ILC) studies covering many different instrument types, commercial and custom-built, as another possible source of variation. ILCs were organized in a consecutive manner starting with dispersions in water employing well-characterized near-spherical silica nanoparticles (nominal 19 nm and 100 nm diameter) and two types of functionalized spherical polystyrene nanoparticles (nominal 50 nm diameter). At first each laboratory used their in-house established procedures. In particular for the 19 nm silica particles, the reproducibility of the methods was unacceptably high (reported results were between 10 nm and 50 nm). When comparing the results of the first ILC round it was observed that the DCS methods performed significantly worse than the DLS methods, thus emphasizing the need for standard operating procedures (SOPs). SOPs have been developed by four expert laboratories but were tested for robustness by a larger number of independent users in a second ILC (11 for DLS and 4 for DCS). In a similar approach another SOP for complex biological fluids, i. e. cell culture medium containing serum was developed, again confirmed via an ILC with 8 participating laboratories. Our study confirms that well-established and fit-for-purpose SOPs are indispensable for obtaining reliable and comparable particle size data. Our results also show that these SOPs must be optimized with respect to the intended measurement system (e. g. particle size technique, type of dispersant) and that they must be sufficiently detailed (e. g. avoiding ambiguity regarding measurand definition, etc. ). SOPs may be developed by a small number of expert laboratories but for their widespread applicability they need to be verified by a larger number of laboratories.
Note: Número d'acord de subvenció EC/FP7/262163
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Language: Anglès.
Document: article ; recerca ; acceptedVersion
Published in: NanoImpact, Vol. 10 (April 2018) , p. 97-107, ISSN 2452-0748

DOI: 10.1016/j.impact.2017.12.004


Available from: 2020-04-30
Postprint

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles

 Record created 2019-09-02, last modified 2019-10-08



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