Web of Science: 25 citations, Scopus: 24 citations, Google Scholar: citations,
Label-free SPR detection of gluten peptides in urine for non-invasive celiac disease follow-up
Soler Aznar, Maria (Institut Català de Nanociència i Nanotecnologia)
Estévez Alberola, Maria Carmen (Institut Català de Nanociència i Nanotecnologia)
Moreno, María de Lourdes (Universidad de Sevilla)
Cebolla, Angel (Biomedal S.L.)
Lechuga, Laura M. (Institut Català de Nanociència i Nanotecnologia)

Date: 2016
Abstract: Motivated by the necessity of new and efficient methods for dietary gluten control of celiac patients, we have developed a simple and highly sensitive SPR biosensor for the detection of gluten peptides in urine. The sensing methodology enables rapid and label-free quantification of the gluten immunogenic peptides (GIP) by using G12 mAb. The overall performance of the biosensor has been in-depth optimized and evaluated in terms of sensitivity, selectivity and reproducibility, reaching a limit of detection of 0. 33ngmL. Besides, the robustness and stability of the methodology permit the continuous use of the biosensor for more than 100 cycles with excellent repeatability. Special efforts have been focused on preventing and minimizing possible interferences coming from urine matrix enabling a direct analysis in this fluid without requiring extraction or purification procedures. Our SPR biosensor has proven to detect and identify gluten consumption by evaluating urine samples from healthy and celiac individuals with different dietary gluten conditions. This novel biosensor methodology represents a novel approach to quantify the digested gluten peptides in human urine with outstanding sensitivity in a rapid and non-invasive manner. Our technique should be considered as a promising opportunity to develop Point-of-Care (POC) devices for an efficient, simple and accurate gluten free diet (GFD) monitoring as well as therapy follow-up of celiac disease patients.
Note: Número d'acord de subvenció MINECO/BES-2010-037704
Note: Número d'acord de subvenció AGAUR/2014/SGR-624
Note: Número d'acord de subvenció MINECO/SEV-2013-0295
Rights: Tots els drets reservats.
Language: Anglès.
Document: article ; recerca ; submittedVersion
Subject: Surface plasmon resonance ; Gluten immunogenic peptides ; 33-mer gliadin peptide ; Gluten detection ; Celiac disease ; POC device
Published in: Biosensors and bioelectronics, Vol. 79 (May 2016) , p. 158-164, ISSN 1873-4235

DOI: 10.1016/j.bios.2015.11.097


Preprint
15 p, 518.9 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles

 Record created 2019-09-02, last modified 2019-10-07



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