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Página principal > Artículos > Artículos publicados > Interspecies interaction and effect of co-contaminants in an anaerobic dichloromethane-degrading culture |
Fecha: | 2020 |
Resumen: | An anaerobic stable mixed culture dominated by bacteria belonging to the genera Dehalobacterium, Acetobacterium, Desulfovibrio, and Wolinella was used as a model to study the microbial interactions during DCM degradation. Physiological studies indicated that DCM was degraded in this mixed culture at least in a three-step process: i) fermentation of DCM to acetate and formate, ii) formate oxidation to CO₂ and H₂, and iii) H₂/CO₂ reductive acetogenesis. The 16S rRNA gene sequencing of cultures enriched with formate or H₂ showed that Desulfovibrio was the dominant population followed by Acetobacterium, but sequences representing Dehalobacterium were only present in cultures amended with DCM. Nuclear magnetic resonance analyses confirmed that acetate produced from ¹³C-labelled DCM was marked at the methyl ([2-¹³C]acetate), carboxyl ([1-¹³C]acetate), and both ([1,2-¹³C]acetate) positions, which is in accordance to acetate formed by both direct DCM fermentation and H₂/CO₂ acetogenesis. The inhibitory effect of ten different co-contaminants frequently detected in groundwaters on DCM degradation was also investigated. Complete inhibition of DCM degradation was observed when chloroform, perfluorooctanesulfonic acid, and diuron were added at 838, 400, and 107 μM, respectively. However, the inhibited cultures recovered the DCM degradation capability when transferred to fresh medium without co-contaminants. Findings derived from this work are of significant relevance to provide a better understanding of the synergistic interactions among bacteria to accomplish DCM degradation as well as to predict the effect of co-contaminants during anaerobic DCM bioremediation in groundwater. |
Ayudas: | Ministerio de Economía y Competitividad CTM2016-75587-C2-1-R Ministerio de Economía y Competitividad BES-2014-070817 Ministerio de Economía y Competitividad CTM2013-48545-C2-1-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-14 |
Derechos: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió acceptada per publicar |
Materia: | Dehalobacterium ; Dichloromethane ; Co-contaminants ; Inhibition ; Bioremediation |
Publicado en: | Chemosphere, Vol. 240 (Feb. 2020) , art. 124877, ISSN 1879-1298 |
Postprint 34 p, 841.1 KB |