Towards the design of universal immunosurfaces for SPR-based assays : a review
Mauriz, Elba (Universidad de León)
García Fernández, M. C. (Universidad de León)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)

Date:	2016
Abstract:	Surface biofunctionalization, including chemical activation and attachment of the bioreceptor, is an essential step to provide reliable detection of biomolecular binding events monitored by Surface Plasmon Resonance (SPR), the most employed optical biosensor, and other biosensor techniques. Recent progress in the area of immobilization procedures are aimed at producing reproducible interfacial surfaces that enable the sensitive and specific recognition of the analyte. Antibodies are still the most employed bioreceptors for SPR assays. A wide range of strategies have been proposed to maximize the SPR immunosensor performance by controlling the stability and orientation of the immobilized antibody. This article reviews the most recent advancements in random and oriented antibody immobilization approaches for SPR biosensing applications, with a special focus on the research that have been done to find universal linkers, which can allow the use of the same functionalized surface for different applications.
Grants:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-624 Ministerio de Economía y Competitividad SEV-2013-0295
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	SPR ; Biofunctionalization ; Antibody immobilization ; Antibody orientation strategies ; Special immunmosurfaces
Published in:	TrAC - Trends in Analytical Chemistry, Vol. 79 (May 2016) , p. 191-198, ISSN 1879-3142

DOI: 10.1016/j.trac.2016.02.006

Postprint 27 p, 1.2 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles