Web of Science: 2 citations, Scopus: 2 citations, Google Scholar: citations,
Cryotolerance of stallion spermatozoa relies on aquaglyceroporins rather than orthodox aquaporins
Delgado-Bermúdez, Ariadna. (Universitat de Girona. Departament de Biologia)
Noto, Federico (Universitat de Girona. Departament de Biologia)
Bonilla-Correal, Sebastián (Universitat Autònoma de Barcelona. Departament de Cirurgia i Medicina Animals)
Garcia-Bonavila, Estela (Universitat de Girona. Departament de Biologia)
Catalán, Jaime (Universitat Autònoma de Barcelona. Departament de Cirurgia i Medicina Animals)
Papas, Marion (Universitat Autònoma de Barcelona. Departament de Cirurgia i Medicina Animals)
Bonet, Sergi (Universitat de Girona. Departament de Biologia)
Miró, Jordi (Equine Reproduction Service. Department of Animal Medicine and Surgery. Faculty of Veterinary Sciences. Autonomous University of Barcelona)
Yeste, Marc (Universitat de Girona. Departament de Biologia)

Date: 2019
Abstract: Aquaporins (AQPs), a family of ubiquitous water channels divided into orthodox AQPs, aquaglyceroporins (GLPs), and superAQPs, are present in stallion spermatozoa. The aim of this study was to elucidate the functional relevance of each group of AQPs during stallion sperm cryopreservation through the use of three different inhibitors: acetazolamide (AC), phloretin (PHL) and propanediol (PDO). Sperm quality and function parameters were evaluated in the presence or absence of each inhibitor in fresh and frozen-thawed samples. In the presence of AC, different parameters were altered (p < 0. 05), but not in a concentration-or time-depending manner. PHL was found to decrease sperm motility, viability, acrosome integrity, and the percentages of spermatozoa with low membrane lipid disorder, high mitochondrial membrane potential (MMP) and high intracellular levels of calcium and superoxides (p < 0. 05). Finally, the sperm motility, viability, acrosome integrity, the percentages of spermatozoa with low membrane lipid disorder, high MMP and high intracellular calcium levels were higher (p < 0. 05) in PDO treatments than in the control. The sperm response to AC, PHL and PDO indicates that GLPs, rather than orthodox AQPs, play a crucial role during stallion sperm cryopreservation. Furthermore, post-thaw sperm quality was higher in PDO treatments than in the control, suggesting that this molecule is a potential permeable cryoprotectant.
Note: Número d'acord de subvenció Regional Government of Catalonia, Spain/2017-SGR-1229
Note: Número d'acord de subvenció MINECO/AGL2017-88329-R
Note: Número d'acord de subvenció MINECO/PRE2018-083488
Note: Número d'acord de subvenció MINECO/RYC-2014-15581
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: article ; recerca ; publishedVersion
Subject: Acetazolamide ; Aquaporins ; Phloretin ; Propanediol ; Sperm ; Stallion
Published in: Biology, Vol. 8 Núm. 4 (december 2019) , p. 85, ISSN 2079-7737

DOI: 10.3390/biology8040085
PMID: 31726707


18 p, 1.6 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2020-01-14, last modified 2020-08-02



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