Structural lesions of proteins connected to lipid membrane damages caused by radical stress : assessment by biomimetic systems and raman spectroscopy
Torreggiani, Armida (CNR-Istituto per la Sintesi Organica e la Fotoreattività)
Tinti, Anna (Università di Bologna. Dipartimento di Scienze Biomediche e Neuromotorie)
Jurasekova, Zuzana (P.J. Šafárik University. Department of Biophysics)
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química)
Saracino, Michela (CNR-Istituto per la Sintesi Organica e la Fotoreattività)
Di Foggia, Michele (Università di Bologna. Dipartimento di Scienze Biomediche e Neuromotorie)

Fecha:	2019
Resumen:	Model systems constituted by proteins and unsaturated lipid vesicles were used to gain more insight into the effects of the propagation of an initial radical damage on protein to the lipid compartment. The latter is based on liposome technology and allows measuring the trans unsaturated fatty acid content as a result of free radical stress on proteins. Two kinds of sulfur-containing proteins were chosen to connect their chemical reactivity with membrane lipid transformation, serum albumins and metallothioneins. Biomimetic systems based on radiation chemistry were used to mimic the protein exposure to different kinds of free radical stress and Raman spectroscopy to shed light on protein structural changes caused by the free radical attack. Among the amino acid residues, Cys is one of the most sensitive residues towards the attack of free radicals, thus suggesting that metal-Cys clusters are good interceptors of reactive species in metallothioneins, together with disulfides moieties in serum albumins. Met is another important site of the attack, in particular under reductive conditions. Tyr and Phe are sensitive to radical stress too, leading to electron transfer reactions or radical-induced modifications of their structures. Finally, modifications in protein folding take place depending on reactive species attacking the protein.
Nota:	Altres ajuts: This research wa sfunded by the project RM@Schools3.0 of the EIT RawMaterials, GrantNumber 17146
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Free radical stress ; Lipid domains ; Protein damage ; Raman spectroscopy ; Desulfurization ; Protein:lipid interaction ; Cis-trans isomerization ; Tandem lesions ; Thiyl radicals
Publicado en:	Biomolecules, Vol. 9, issue 12 (Dec. 2019) , art. 794, ISSN 2218-273X

DOI: 10.3390/biom9120794
PMID: 31783702

18 p, 3.1 MB

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