Web of Science: 1 citations, Scopus: 1 citations, Google Scholar: citations
Zymobacter palmae pyruvate decarboxylase production process development : Cloning in Escherichia coli, fed-batch culture and purification
Alcover, Natàlia (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Carceller, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Álvaro Campos, Gregorio (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Guillén Montalbán, Marina (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Date: 2019
Abstract: Pyruvate decarboxylase (PDC) is responsible for the decarboxylation of pyruvate, producing acetaldehyde and carbon dioxide and is of high interest for industrial applications. PDC is a very powerful tool in the enzymatic synthesis of chiral amines by combining it with transaminases when alanine is used as amine donor. However, one of the main drawback that hampers its use in biocatalysis is its production and the downstream processing on scale. In this paper, a production process of PDC from Zymobacter palmae has been developed. The enzyme has been cloned and overexpressed in Escherichia coli. It is presented, for the first time, the evaluation of the production of recombinant PDC in a bench-scale bioreactor, applying a substrate-limiting fed-batch strategy which led to a volumetric productivity and a final PDC specific activity of 6942 U L−1h−1 and 3677 U gDCW−1 (dry cell weight). Finally, PDC was purified in fast protein liquid chromatography equipment by ion exchange chromatography. The developed purification process resulted in 100% purification yield and a purification factor of 3. 8.
Note: Número d'acord de subvenció MINECO/CTQ2014-53114-R
Note: Número d'acord de subvenció AGAUR/2017/SGR-1462
Note: Número d'acord de subvenció MINECO/BES-2015-072542
Rights: Tots els drets reservats
Language: Anglès
Document: article ; recerca ; acceptedVersion
Subject: Fed-batch culture ; High cell density cultures ; PDC purification ; Pyruvate decarboxylase (PDC) ; Zymobacter palmae
Published in: Engineering in life science, Vol. 19, Issue 7 (July 2019) , p. 502-512, ISSN 1618-0240

DOI: 10.1002/elsc.201900010
PMID: 32625027


Postprint
33 p, 1.6 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2020-01-21, last modified 2020-08-02



   Favorit i Compartir