Cerium oxide nanoparticles improve liver regeneration after acetaminophen-induced liver injury and partial hepatectomy in rats
Cordoba, Bernat (Hospital Clínic i Provincial de Barcelona)
Arce Cerezo, Altamira (Hospital Clínic i Provincial de Barcelona)
Ribera, Jordi (Hospital Clínic i Provincial de Barcelona)
Pauta, Montse (Hospital Clínic i Provincial de Barcelona)
Oró, Denise (Hospital Clínic i Provincial de Barcelona)
Casals, Gregori (Hospital Clínic i Provincial de Barcelona)
Fernández-Varo, Guillermo (Hospital Clínic i Provincial de Barcelona)
Casals, Eudald (Institut Català de Nanociència i Nanotecnologia)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
Jiménez, Wladimiro (Universitat de Barcelona. Departament de Biomedicina)
Morales Ruiz, Manuel (Universitat de Barcelona. Departament de Biomedicina)

Fecha:	2019
Resumen:	Background and aims: Cerium oxide nanoparticles are effective scavengers of reactive oxygen species and have been proposed as a treatment for oxidative stress-related diseases. Consequently, we aimed to investigate the effect of these nanoparticles on hepatic regeneration after liver injury by partial hepatectomy and acetaminophen overdose. Methods: All the in vitro experiments were performed in HepG2 cells. For the acetaminophen and partial hepatectomy experimental models, male Wistar rats were divided into three groups: (1) nanoparticles group, which received 0. 1 mg/kg cerium nanoparticles i. v. twice a week for 2 weeks before 1 g/kg acetaminophen treatment, (2) N-acetyl-cysteine group, which received 300 mg/kg of N-acetyl-cysteine i. p. 1 h after APAP treatment and (3) partial hepatectomy group, which received the same nanoparticles treatment before partial hepatectomy. Each group was matched with vehicle-controlled rats. Results: In the partial hepatectomy model, rats treated with cerium oxide nanoparticles showed a significant increase in liver regeneration, compared with control rats. In the acetaminophen experimental model, nanoparticles and N-acetyl-cysteine treatments decreased early liver damage in hepatic tissue. However, only the effect of cerium oxide nanoparticles was associated with a significant increment in hepatocellular proliferation. This treatment also reduced stress markers and increased cell cycle progression in hepatocytes and the activation of the transcription factor NF-κB in vitro and in vivo. Conclusions: Our results demonstrate that the nanomaterial cerium oxide, besides their known antioxidant capacities, can enhance hepatocellular proliferation in experimental models of liver regeneration and drug-induced hepatotoxicity.
Ayudas:	Ministerio de Ciencia e Innovación SAF2016‑75358‑R
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Liver regeneration ; Oxidative stress ; Cerium oxide nanoparticles ; Partial hepatectomy ; Acetaminophen-induced liver injury
Publicado en:	Journal of nanobiotechnology, Vol. 17 (October 2019) , art. 112, ISSN 1477-3155

DOI: 10.1186/s12951-019-0544-5
PMID: 31672158

12 p, 4.4 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Institut Català de Nanociència i Nanotecnologia (ICN2)
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