Distinct pathways for zinc metabolism in the terrestrial slug Arion vulgaris
Dvorak, Martin (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences)
Schnegg, Raimund (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences)
Salvenmoser, Willy (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences)
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química)
Lindner, Herbert (Innsbruck Medical University. Institute of Clinical Biochemistry. Biocenter)
Zerbe, Oliver (University of Zurich. Department of Chemistry)
Hansel, Armin (University of Innsbruck. Institute for Ion Physics and Applied Physics)
Leiminger, Markus (University of Innsbruck. Institute for Ion Physics and Applied Physics)
Steiner, Gerhard (University of Innsbruck. Institute for Ion Physics and Applied Physics)
Dallinger, Reinhard (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences)
Lackner, Reinhard (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences)

Date:	2019
Abstract:	In most organisms, the concentration of free Zn is controlled by metallothioneins (MTs). In contrast, no significant proportions of Zn are bound to MTs in the slug, Arion vulgaris. Instead, this species possesses cytoplasmic low-molecular-weight Zn (LMW Zn) binding compound that divert these metal ions into pathways uncoupled from MT metabolism. Zn is accumulated in the midgut gland calcium cells of Arion vulgaris, where they associate with a low-molecular-weight ligand with an apparent molecular mass of ~ 2,000 Da. Mass spectrometry of the semi-purified LMW Zn binding compound combining an electrospray ion source with a differential mobility analyser coupled to a time-of-flight mass spectrometer revealed the presence of four Zn-containing ion signals, which arise from disintegration of one higher MW complex resulting in an ion-mobility diameter of 1. 62 nm and a molecular mass of 837 Da. We expect that the novel Zn ion storage pathway may be shared by many other gastropods, and particularly species that possess Cd-selective MT isoforms or variants with only very low affinity to Zn.
Grants:	Ministerio de Economía y Competitividad BIO2015-67358-C2-2-P Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-864
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Scientific reports, Vol. 9 (December 2019) , art. 20089, ISSN 2045-2322

DOI: 10.1038/s41598-019-56577-7
PMID: 31882936

10 p, 2.0 MB

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