Size-dependent protein-nanoparticle interactions in citrate-stabilized gold nanoparticles : the emergence of the protein corona
Piella, Jordi (Institut Català de Nanociència i Nanotecnologia)
Bastús, Neus G. (Institut Català de Nanociència i Nanotecnologia)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)

Date:	2017
Abstract:	Surface modifications of highly monodisperse citrate-stabilized gold nanoparticles (AuNPs) with sizes ranging from 3. 5 to 150 nm after their exposure to cell culture media supplemented with fetal bovine serum were studied and characterized by the combined use of UV-vis spectroscopy, dynamic light scattering, and zeta potential measurements. In all the tested AuNPs, a dynamic process of protein adsorption was observed, evolving toward the formation of an irreversible hard protein coating known as Protein Corona. Interestingly, the thickness and density of this protein coating were strongly dependent on the particle size, making it possible to identify different transition regimes as the size of the particles increased: (i) NP-protein complexes (or incomplete corona), (ii) the formation of a near-single dense protein corona layer, and (iii) the formation of a multilayer corona. In addition, the different temporal patterns in the evolution of the protein coating came about more quickly for small particles than for the larger ones, further revealing the significant role that size plays in the kinetics of this process. Since the biological identity of the NPs is ultimately determined by the protein corona and different NP-biological interactions take place at different time scales, these results are relevant to biological and toxicological studies.
Grants:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-612 Ministerio de Economía y Competitividad RYC-2012-10991 European Commission 322153 Ministerio de Ciencia e Innovación MAT2015-70725-R
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Subject:	Adsorption ; Citrates ; Gold ; Nanoparticles ; Particle Size ; Proteins ; Spectrophotometry, Ultraviolet ; Surface Properties
Published in:	Bioconjugate chemistry, Vol. 28, Issue 1 (January 2017) , p. 88-97, ISSN 1520-4812

DOI: 10.1021/acs.bioconjchem.6b00575

Preprint 25 p, 2.8 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles