Electrochemically reduced graphene and iridium oxide nanoparticles for inhibition-based angiotensin-converting enzyme inhibitor detection
Kurbanoglu, Sevinc (Institut Català de Nanociència i Nanotecnologia)
Rivas, Lourdes (Institut Català de Nanociència i Nanotecnologia)
Ozkan, Sibel A. (Ankara University. Department of Analytical Chemistry)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Fecha:	2017
Resumen:	In this work, a novel biosensor based on electrochemically reduced graphene oxide and iridium oxide nanoparticles for the detection of angiotensin-converting enzyme inhibitor drug, captopril, is presented. For the preparation of the biosensor, tyrosinase is immobilized onto screen printed electrode by using 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-Hydroxysuccinimide coupling reagents, in electrochemically reduced graphene oxide and iridium oxide nanoparticles matrix. Biosensor response is characterized towards catechol, in terms of graphene oxide concentration, number of cycles to reduce graphene oxide, volume of iridium oxide nanoparticles and tyrosinase solution. The designed biosensor is used to inhibit tyrosinase activity by Captopril, which is generally used to treat congestive heart failure. It is an angiotensin-converting enzyme inhibitor that operates via chelating copper at the active site of tyrosinase and thioquinone formation. The captopril detections using both inhibition ways are very sensitive with low limits of detection: 0. 019 µM and 0. 008 µM for chelating copper at the active site of tyrosinase and thioquinone formation, respectively. The proposed methods have been successfully applied in captopril determination in spiked human serum and pharmaceutical dosage forms with acceptable recovery values.
Ayudas:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-260 Ministerio de Economía y Competitividad SEV-2013-0295 Ministerio de Economía y Competitividad MAT2014-52485-P
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Captopril detection ; Electrochemically reduced graphene oxide ; Enzyme inhibition ; Iridium oxide nanoparticles ; Angiotensin-converting enzyme inhibitor ; Enzyme biosensors
Publicado en:	Biosensors & bioelectronics, Vol. 88 (February 2017) , p. 122-129, ISSN 1873-4235

DOI: 10.1016/j.bios.2016.07.109

Postprint 33 p, 1.1 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Institut Català de Nanociència i Nanotecnologia (ICN2)
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