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Effects of Cannabis Use on the Protein and Lipid Profile of Olfactory Neuroepithelium Cells from Schizophrenia Patients Studied by Synchrotron-Based FTIR Spectroscopy
Saladrigas Manjón, Sergi (Institut Hospital del Mar d'Investigacions Mèdiques)
Dučić, Tanja (ALBA Laboratori de Llum de Sincrotró)
Galindo Guarin, Liliana (University of Cambridge. Department of Psychiatry)
Fernández Avilés, Cristina (Institut Hospital del Mar d'Investigacions Mèdiques)
Pérez, Víctor (Universitat Autònoma de Barcelona. Departament de Psiquiatria i Medicina Legal)
de la Torre, Rafael (Institut Hospital del Mar d'Investigacions Mèdiques)
Robledo, Patricia (Institut Hospital del Mar d'Investigacions Mèdiques)

Date: 2020
Abstract: Schizophrenia (SCZ) is a neurodevelopmental disorder with a high genetic component, but the presence of environmental stressors can be important for its onset and progression. Cannabis use can be a major risk factor for developing SCZ. However, despite the available data on the neurobiological underpinnings of SCZ, there is an important lack of studies in human neuronal tissue and living cells addressing the effects of cannabis in SCZ patients. In this study, we analysed the most relevant bio-macromolecular constituents in olfactory neuroepithelium (ON) cells of healthy controls non-cannabis users, healthy cannabis users, SCZ patients non-cannabis users, and SCZ patients cannabis users using Synchrotron Radiation-Fourier Transform Infrared (SR-FTIR) spectrometry and microscopy. Our results revealed that SCZ patients non-cannabis users, and healthy cannabis users exhibit similar alterations in the macromolecular profile of ON cells, including disruption in lipid composition, increased lipid membrane renewal rate and lipid peroxidation, altered proteins containing more β-sheet structures, and showed an increase in DNA and histone methylation. Notably, these alterations were not observed in SCZ patients who use cannabis regularly. These data suggest a differential effect of cannabis in healthy controls and in SCZ patients in terms of the macromolecular constituents of ON cells.
Note: Número d'acord de subvenció ISCIII/PI14-00210
Note: Número d'acord de subvenció ISCIII/PI18-00053
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: article ; publishedVersion
Subject: Schizophrenia ; Olfactory neuroepithelium ; SR-FTIR ; Cannabis
Published in: Biomolecules, Vol. 10, Issue 2 (February 2020) , art. 329, ISSN 2218-273X

DOI: 10.3390/biom10020329
PMID: 32092878

16 p, 1.7 MB

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Articles > Published articles

 Record created 2020-07-06, last modified 2020-08-02

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