Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting
Dopico, Eva (Institut Català de la Salut)
Del-Rei, Rodrigo Pimenta (Faculty of Technology and Sciences of Bahia)
Espinoza, Bertha (Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas)
Ubillos, Itziar (Institut Català de la Salut)
Zanchin, Nilson Ivo Tonin (Oswaldo Cruz Foundation. Carlos Chagas Institute)
Sulleiro Igual, Elena (Hospital Universitari Vall d'Hebron)
Moure García, Zaira (Hospital Universitari Vall d'Hebron)
Celedon, Paola Alejandra Fiorani (Molecular Biology Institute of Paraná)
Souza, Wayner Vieira (Oswaldo Cruz Foundation. Aggeu Magalhães Institute)
da Silva, Edimilson Domingos (Oswaldo Cruz Foundation. Immunobiological Technology Institute)
Gomes, Yara Miranda (Oswaldo Cruz Foundation. Aggeu Magalhães Institute)
Santos, Fred Luciano Neves (Oswaldo Cruz Foundation. Gonçalo Moniz Institute)
Universitat Autònoma de Barcelona

Fecha:	2019
Resumen:	Chronic Chagas Disease (CD) diagnosis is based on serological methods employing crude, semipurified or recombinant antigens, which may result in low sensitivity or cross-reactivity. To reduce these restrictions, we developed a strategy involving use of molecules containing repetitive fragments of Trypanosoma cruzi conserved proteins. Diagnostic performance of IBMP-8. 1 and IBMP-8. 4 chimeric antigens (Molecular Biology Institute of Paraná - IBMP in Portuguese acronym) was assessed to diagnose T. cruzi -infected and non-infected immigrants living in Barcelona (Spain), a non-endemic setting for Chagas disease. Reactivity of IBMP-8. 1 and IBMP-8. 4 was assessed using an in-house automated ELISA with 347 positive and 331 negative individuals to Chagas disease. Antigenic cross-reactivity was measured with sera samples from pregnant women with Toxoplasma gondii (n = 98) and Zika virus (n = 75) antibodies. The area under the curve values was 1 and 0. 99 for the IBMP-8. 1 and IBMP-8. 4 proteins, respectively, demonstrating excellent diagnostic accuracy. The reactivity index was higher for IBMP-8. 1 than IBMP-8. 4 in positive samples and no significant difference in reactivity index was observed in negative samples. Sensitivity ranged from 99. 4% for IBMP-8. 1 to 99. 1% for IBMP-8. 4 and was not statistically different. Specificity for IBMP-8. 1 reached 100 and 99. 7% for IBMP-8. 4, both nearly 100% accurate. No antigenic cross-reactivity was observed and reactivity index was similar to that for negative Chagas disease individuals. Our results showed an outstanding performance of IBMP-8. 1 and IBMP-8. 4 chimeric antigens by ELISA and suggest both chimeric antigens could also be used for Chagas disease diagnosis in immigrants living in non-endemic settings.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Chagas disease ; Trypanosoma cruzi ; Chimeric antigens ; Immunoassay ; Accuracy
Publicado en:	BMC Infectious diseases, Vol. 19 (march 2019) , ISSN 1471-2334

DOI: 10.1186/s12879-019-3872-z
PMID: 30871504

7 p, 1.2 MB

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