Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens
Chaves Hernández, Aida Jeannette (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Busquets, Núria (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Valle, Rosa (Institut de Recerca i Tecnologia Agroalimentàries)
Rivas, Raquel (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Vergara-Alert, Júlia (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Dolz, Roser (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Ramis Salva, Antonio José (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Darji, Ayub (Institut de Recerca i Tecnologia Agroalimentàries)
Majó i Masferrer, Natàlia (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)

Fecha:	2011
Resumen:	In order to understand the mechanism of neuroinvasion of a highly pathogenic avian influenza virus (HPAIV) into the central nervous system (CNS) of chickens, specific pathogen free chickens were inoculated with a H7N1 HPAIV. Blood, cerebrospinal fluid (CSF), nasal cavity and brain tissue samples were obtained from 1 to 4 days post-inoculation (dpi) of infected and control chickens. Viral antigen topographical distribution, presence of influenza A virus receptors in the brain, as well as, the role of the olfactory route in virus CNS invasion were studied using different immunohistochemistry techniques. Besides, viral RNA load in CSF and blood was quantified by means of a quantitative real-time reverse transcription-polymerase chain reaction. Viral antigen was observed widely distributed in the CNS, showing bilateral and symmetrical distribution in the nuclei of the diencephalon, mesencephalon and rhombencephalon. Viral RNA was detected in blood and CSF at one dpi, indicating that the virus crosses the blood-CSF-barrier early during infection. This early dissemination is possibly favoured by the presence of Siaα2,3 Gal and Siaα2,6 Gal receptors in brain vascular endothelial cells, and Siaα2,3 Gal receptors in ependymal and choroid plexus cells. No viral antigen was observed in olfactory sensory neurons, while the olfactory bulb showed only weak staining, suggesting that the virus did not use this pathway to enter into the brain. The sequence of virus appearance and the topographical distribution of this H7N1 HPAIV indicate that the viral entry occurs via the haematogenous route, with early and generalized spreading through the CSF.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; Versió publicada
Materia:	Grip aviària ; Avian influenza ; Sistema nerviós ; Nervous system
Publicado en:	Veterinary research, Vol. 42 (octubre 2011) , p. 106, ISSN 1297-9716

Adreça alternativa: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199250/
Adreça alternativa: http://www.veterinaryresearch.org/content/42/1/106
DOI: 10.1186/1297-9716-42-106
PMID: 21982125

13 p, 13.6 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
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