Web of Science: 3 citas, Scopus: 3 citas, Google Scholar: citas,
A compact SPR biosensor device for the rapid and efficient monitoring of gluten-free diet directly in human urine
Peláez Gutiérrez, Enelia Cristina (Institut Català de Nanociència i Nanotecnologia)
Estévez, M. Carmen (Institut Català de Nanociència i Nanotecnologia)
Domínguez, Remedios (Biomedal S.L)
Sousa, Carolina (Universidad de Sevilla)
Cebolla, Angel (Biomedal S.L)
Lechuga, Laura (Institut Català de Nanociència i Nanotecnologia)

Título variante: A compact plasmonic biosensor device for the rapid and efficient monitoring of gluten free diet directly in human urine
Fecha: 2020
Resumen: Celiac disease (CD) is a chronic autoimmune disorder induced in genetically susceptible individuals by the ingestion of gluten from wheat, rye, barley, or certain varieties of oats. A careful diet follow-up is necessary to avoid health complications associated with long-term gluten intake by the celiac patients. Small peptides (GIP, gluten immunogenic peptides) derived from gluten digestion, which are excreted in the urine and feces, have emerged as promising biomarkers to monitor gluten intake. We have implemented a simple and sensitive label-free point-of-care (POC) device based on surface plasmon resonance for the direct detection of these biomarkers in urine. The assay employs specific monoclonal antibodies and has been optimized for the detection of the 33-mer α2-gliadin, known as the main immunogenic peptide of wheat gluten, and for the detection of GIP. Direct detection in undiluted urine has been accomplished by using biosensing chips containing a robust and stable biorecognition layer, obtained after carefully optimizing the biofunctionalization protocol. Excellent limits of detection have been reached (1. 6-4. 0 ng mL using mAb G12 and A1, respectively), which ensures the detection of gluten peptides even when the gluten intake is around the maximum tolerable amount in the digestive tract (< 50 mg) for celiac individuals. No sample pretreatment, extraction, or dilution is required, and the analysis takes less than 15 min. The assays have excellent reproducibility‚ as demonstrated by measuring spiked urine samples containing the same target concentration using different biofunctionalized chips prepared and stored at different periods of time (i. e. , CV% of 3. 58% and 11. 30%, for G12- and A1-based assays, respectively). The assay has been validated with real samples. These features pave the way towards an end-user easy-to-handle biosensor device for the rapid monitoring of gluten-free diet (GFD) and follow-up of the health status in celiac patients.
Nota: Número d'acord de subvenció MINECO/SEV-2017-0706
Derechos: Tots els drets reservats.
Lengua: Anglès
Documento: article ; recerca ; submittedVersion
Materia: POC device ; Gluten immunogenic peptides ; 33-mer gliadin peptide ; Celiac disease ; Urine ; Gluten-free diet
Publicado en: Analytical and bioanalytical chemistry, Vol. 412 (April 2020) , p. 6407-6417, ISSN 1618-2650

DOI: 10.1007/s00216-020-02616-6


Preprint
32 p, 2.4 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Institut Català de Nanociència i Nanotecnologia (ICN2)
Artículos > Artículos de investigación
Artículos > Artículos publicados

 Registro creado el 2020-07-27, última modificación el 2021-03-03



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