Tutorial : design and fabrication of nanoparticle-based lateral-flow immunoassays
Parolo, Claudio (Institut Català de Nanociència i Nanotecnologia)
Sena Torralba, Amadeo (Institut Català de Nanociència i Nanotecnologia)
Bergua Canudo, José Francisco (Institut Català de Nanociència i Nanotecnologia)
Calucho, Enric (Institut Català de Nanociència i Nanotecnologia)
Fuentes-Chust, Celia (Institut Català de Nanociència i Nanotecnologia)
Hu, Liming (Institut Català de Nanociència i Nanotecnologia)
Rivas, Lourdes (Institut Català de Nanociència i Nanotecnologia)
Álvarez Diduk, Ruslan (Institut Català de Nanociència i Nanotecnologia)
Nguyen, Emily P. (Institut Català de Nanociència i Nanotecnologia)
Cinti, Stefano (Università degli Studi di Napoli Federico II)
Quesada-González, Daniel (Paperdrop Diagnostics)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Fecha:	2020
Resumen:	Lateral-flow assays (LFAs) are quick, simple and cheap assays to analyze various samples at the point of care or in the field, making them one of the most widespread biosensors currently available. They have been successfully employed for the detection of a myriad of different targets (ranging from atoms up to whole cells) in all type of samples (including water, blood, foodstuff and environmental samples). Their operation relies on the capillary flow of the sample throughout a series of sequential pads, each with different functionalities aiming to generate a signal to indicate the absence/presence (and, in some cases, the concentration) of the analyte of interest. To have a user-friendly operation, their development requires the optimization of multiple, interconnected parameters that may overwhelm new developers. In this tutorial, we provide the readers with: (i) the basic knowledge to understand the principles governing an LFA and to take informed decisions during lateral flow strip design and fabrication, (ii) a roadmap for optimal LFA development independent of the specific application, (iii) a step-by-step example procedure for the assembly and operation of an LF strip for the detection of human IgG and (iv) an extensive troubleshooting section addressing the most frequent issues in designing, assembling and using LFAs. By changing only the receptors, the provided example procedure can easily be adapted for cost-efficient detection of a broad variety of targets.
Ayudas:	European Commission 825694 European Commission 795635 European Commission 785219 European Commission 754510 Ministerio de Economía y Competitividad SEV-2017-0706 Ministerio de Ciencia e Innovación PRE2018-084856
Nota:	Altres ajuts: ICN2 is funded by the CERCA programme/Generalitat de Catalunya.
Derechos:	Tots els drets reservats.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Publicado en:	Nature Protocols, Vol. 15, issue 12 (Dec. 2020) , p. 3788-3816, ISSN 1750-2799

DOI: 10.1038/s41596-020-0357-x

Postprint 59 p, 2.6 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Institut Català de Nanociència i Nanotecnologia (ICN2)
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