Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular Acinetobacter baumannii
Crua Asensio, Núria (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Macho Rendón, Javier (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Torrent Burgas, Marc (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Date:	2021
Abstract:	The rise in the number of antibiotic-resistant bacteria has become a serious threat to health, making it important to identify, characterize and optimize new molecules to help us to overcome the infections they cause. It is well known that Acinetobacter baumannii has a significant capacity to evade the actions of antibacterial drugs, leading to its emergence as one of the bacteria responsible for hospital and community-acquired infections. Nonetheless, how this pathogen infects and survives inside the host cell is unclear. In this study, we analyze the time-resolved transcriptional profile changes observed in human epithelial HeLa cells after infection by A. baumannii, demonstrating how it survives in host cells and starts to replicate 4 h post infection. These findings were achieved by sequencing RNA to obtain a set of Differentially Expressed Genes (DEGs) to understand how bacteria alter the host cells' environment for their own benefit. We also determine common features observed in this set of genes and identify the protein-protein networks that reveal highly-interacted proteins. The combination of these findings paves the way for the discovery of new antimicrobial candidates for the treatment of multidrug-resistant bacteria.
Grants:	Ministerio de Economía y Competitividad SAF2015-72518-EXP Agencia Estatal de Investigación SAF2017-82158-R Ministerio de Economía y Competitividad RYC-2012-09999 Agencia Estatal de Investigación SAF2017-82158-R
Note:	Altres ajuts: European Society of Clinical Microbiology and Infectious Diseases Research Grant 2016
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Acinetobacter baumannii ; Infection ; Intracellular ; Transcriptional profile
Published in:	Microorganisms, Vol. 9, Issue 2 (February 2021) , art. 354, ISSN 2076-2607

DOI: 10.3390/microorganisms9020354
PMID: 33670223

14 p, 2.0 MB

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