Novel Use of PLGA Microspheres to Create an Animal Model of Glaucoma with Progressive Neuroretinal Degeneration
Garcia-Herranz, David (Universidad Complutense de Madrid. Departamento de Farmacia Gelénica y Tecnología Alimentaria)
Rodrigo Sanjuan, María Jesús (Instituto de Investigación Sanitaria de Aragón)
Subías, Manuel (Instituto de Investigación Sanitaria de Aragón)
Martínez-Rincón, Teresa (Instituto de Investigación Sanitaria de Aragón)
Mendez-Martínez, Silvia (Instituto de Investigación Sanitaria de Aragón)
Bravo-Osuna, Irene (Universidad Complutense de Madrid. Instituto Universitario de Farmacia Industrial)
Bonet, Aina (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Ruberte, Jesús (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
García-Feijoo, Julián (Universidad Complutense de Madrid. Departamento de Inmunología, Oftalmología y Otorrinolaringología)
Pablo, Luis E (Instituto de Investigación Sanitaria de Aragón)
Garcia-Martin, Elena (Instituto de Investigación Sanitaria de Aragón)
Herrero-Vanrell, Rocío (Universidad Complutense de Madrid. Instituto Universitario de Farmacia Industrial)

Fecha:	2021
Resumen:	Progressive degeneration of neuroretinal tissue with maintained elevated intraocular pressure (IOP) to simulate chronic glaucoma was produced by intracameral injections of poly (lactic-co-glycolic) acid (PLGA) microspheres (Ms) in rat eyes. The right eye of 39 rats received different sizes of PLGA-Ms (2 µL suspension; 10% w / v): 14 with 38-20 µm Ms (Ms38/20 model) and 25 with 20-10 µm particles (Ms20/10 model). This novel glaucoma animal model was compared to the episcleral vein sclerosis (EPI) model (25 eyes). Injections were performed at baseline, two, four and six weeks. Clinical signs, IOP, retina and optic nerve thicknesses (using in vivo optical coherence tomography; OCT), and histological studies were performed. An IOP increment was observed in all three groups, however, the values obtained from the PLGA-Ms injection resulted lower with a better preservation of the ocular surface. In fact, the injection of Ms20/10 created a gentler, more progressive, and more sustained increase in IOP. This IOP alteration was correlated with a significant decrease in most OCT parameters and in histological ganglion-cell count for the three conditions throughout the eight-week follow-up. In all cases, progressive degeneration of the retina, retinal ganglion cells and optic nerve, simulating chronic glaucoma, was detected by OCT and corroborated by histological study. Results showed an alternative glaucoma model to the well-known episcleral vein model, which was simpler to perform, more reproducible and easier to monitor in vivo.
Ayudas:	Agencia Estatal de Investigación FEDER/MAT2017-83858-C2-1 Agencia Estatal de Investigación FEDER/MAT2017-83858-C2-2 Ministerio de Economía y Competitividad RD16/0008/0004 Ministerio de Economía y Competitividad RD16/0008/0009 Ministerio de Economía y Competitividad RD16/0008/029
Nota:	Altres ajuts: RHR/M17/00213
Nota:	Altres ajuts: RHR/PI17/01726
Nota:	Altres ajuts: RHR/PI17/01946
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Oftalmologia veterinària ; PLGA microspheres ; Intraocular pressure ; Glaucoma animal model ; Intracameral injection ; Optic nerve ; Neurodegeneration
Publicado en:	Pharmaceutics, Vol. 13 (february 2021) , ISSN 1999-4923

DOI: 10.3390/pharmaceutics13020237
PMID: 33567776

20 p, 7.4 MB

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