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Página principal > Artículos > Artículos publicados > Smart chip for visual detection of bacteria using the electrochromic properties of polyaniline |
Fecha: | 2019 |
Resumen: | Finding fast and reliable ways to detect pathogenic bacteria is crucial for addressing serious public health issues in clinical, environmental, and food settings. Here, we present a novel assay based on the conversion of an electrochemical signal into a more convenient optical readout for the visual detection of Escherichia coli. Electropolymerizing polyaniline (PANI) on an indium tin oxide screen-printed electrode (ITO SPE), we achieved not only the desired electrochromic behavior but also a convenient way to modify the electrode surface with antibodies (taking advantage of the many amine groups of PANI). Applying a constant potential to the PANI-modified ITO SPE induces a change in their oxidation state, which in turn generates a color change on the electrode surface. The presence of E. coli on the electrode surface increases the resistance in the circuit affecting the PANI oxidation states, producing a different electrochromic response. Using this electrochromic sensor, we could measure concentrations of E. coli spanning 4 orders of magnitude with a limit of detection of 10 colony forming unit per 1 mL (CFU mL) by the naked eye and 10 CFU mL using ImageJ software. In this work we show that merging the sensitivity of electrochemistry with the user-friendliness of an optical readout can generate a new and powerful class of biosensors, with potentially unlimited applications in a variety of fields. |
Ayudas: | European Commission 795635 Ministerio de Economía y Competitividad SEV-2017-0706 Ministerio de Economía y Competitividad MAT2017-87202-P |
Nota: | Altres ajuts: the ICN2 is funded by the CERCA Programme/Generalitat de Catalunya. |
Derechos: | Tots els drets reservats. |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió acceptada per publicar |
Materia: | Colony forming units ; Electrochemical signals ; Electrochromic behavior ; Electrochromic properties ; Measure concentration ; Pathogenic bacterium ; Public health issues ; Screen printed electrodes |
Publicado en: | Analytical chemistry, Vol. 91, issue 23 (Dec. 2019) , p. 14960-14966, ISSN 1520-6882 |
Postprint 8 p, 1.2 MB |