Consensus Multilocus Sequence Typing Scheme for Pneumocystis jirovecii
Pasic, Lana (Westmead Institute for Medical Research)
Goterris, Lidia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Guerrero Murillo, Mercedes (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Irinyi, Laszlo (Westmead Institute for Medical Research)
Kan, Alex (Westmead Institute for Medical Research)
Ponce, Carolina A. (Instituto de Ciencias Biomédicas)
Vargas, Sergio L. (Instituto de Ciencias Biomédicas)
Martin-Gomez, M. Teresa (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Meyer, Wieland (Westmead Hospital)

Fecha:	2020
Resumen:	Pneumocystis jirovecii is an opportunistic human pathogenic fungus causing severe pneumonia mainly in immunocompromised hosts. Multilocus sequence typing (MLST) remains the gold standard for genotyping of this unculturable fungus. However, the lack of a consensus scheme impedes a global comparison, large scale population studies and the development of a global MLST database. To overcome this problem this study compared all genetic regions (19 loci) currently used in 31 different published Pneumocystis MLST schemes. The most diverse/commonly used eight loci, β-TUB, CYB, DHPS, ITS1, ITS1/2, mt26S and SOD, were further assess for their ability to be successfully amplified and sequenced, and for their discriminatory power. The most successful loci were tested to identify genetically related and unrelated cases. A new consensus MLST scheme consisting of four genetically independent loci: β-TUB, CYB, mt26S and SOD, is herein proposed for standardised P. jirovecii typing, successfully amplifying low and high fungal burden specimens, showing adequate discriminatory power, and correctly identifying suspected related and unrelated isolates. The new consensus MLST scheme, if accepted, will for the first time provide a powerful tool to investigate outbreak settings and undertake global epidemiological studies shedding light on the spread of this important human fungal pathogen.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	P. jirovecii ; Consensus MLST scheme ; β-TUB ; CYB ; Mt26s and SOD
Publicado en:	Journal of Fungi, Vol. 6, Num. 4 (October 2020) , art. 259, ISSN 2309-608X

DOI: 10.3390/jof6040259
PMID: 33143112

20 p, 2.3 MB

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