Towards the standardization of methods of tissue processing for the isolation of mesenchymal stromal cells for clinical use
García Muñoz, Elisabeth (Universitat Autònoma de Barcelona. Banc de Sang i Teixits)
Vives Armengol, Joaquim (Universitat Autònoma de Barcelona. Departament de Medicina)

Fecha:	2021
Resumen:	Multipotent mesenchymal stromal cells (MSCs) are currently the most extensively studied type of adult stem cells in advanced stages of development in the field of regenerative medicine. The biological properties of MSCs have generated great hope for their therapeutic use in degenerative and autoimmune conditions that, at present, lack effective treatment options. Over the last decades, MSCs have been typically obtained from adult bone marrow, but the extraction process is highly invasive and the quality and numbers of isolated cells is drastically influenced by patient age, medication and associated comorbidities. Therefore, there is currently an open discussion on the convenience of allogeneic over autologous treatments, despite potential disadvantages such as rejection by the host. This shift to the allogeneic setting entails the need for high production of MSCs to ensure availability of sufficient cell numbers for transplantation, and therefore making the search for alternative tissue sources of highly proliferative MSC cultures with low levels of senescence occurrence, which is one of the greatest current challenges in the scale up of therapeutic cell bioprocessing. Herein we (i) present the main isolation protocols of MSCs from bone marrow, adipose tissue and Wharton's jelly of the umbilical cord; and (ii) compare their qualities from a bioprocess standpoint, addressing both quality and regulatory aspects, in view of their anticipated clinical use.
Ayudas:	Instituto de Salud Carlos III PI19/01788 Ministerio de Economía y Competitividad RD16/0011/0028 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-719
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Multipotent mesenchymal stromal cells ; Bone marrow ; Wharton's jelly ; Umbilical cord ; Adipose tissue ; MSCs isolation protocols
Publicado en:	Cytotechnology, may 2021, p. 1-10, ISSN 1573-0778

DOI: 10.1007/s10616-021-00474-3
PMID: 33994662

10 p, 471.7 KB

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