Web of Science: 13 citations, Scopus: 12 citations, Google Scholar: citations
Interaction between macrophages and nanoparticles : in vitro 3d cultures for the realistic assessment of inflammatory activation and modulation of innate memory
Swartzwelter, Benjamin (Consiglio Nazionale delle Ricerche. Istituto di Biochimica e Biologia Cellulare)
Verde, Alessandro (Consiglio Nazionale delle Ricerche. Istituto di Biochimica e Biologia Cellulare)
Rehak, Laura (Athena Biomedical Innovations (Roma, Itàlia))
Madej, Mariusz (Consiglio Nazionale delle Ricerche. Istituto di Biochimica e Biologia Cellulare)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
De Luca, Anna Chiara (Consiglio Nazionale delle Ricerche. Istituto di Biochimica e Biologia Cellulare)
Boraschi, Diana (Consiglio Nazionale delle Ricerche. Istituto di Biochimica e Biologia Cellulare)
Italiani, Paola (Stazione Zoologica Anton Dohrn)

Date: 2021
Abstract: Understanding the modes of interaction between human monocytes/macrophages and engineered nanoparticles is the basis for assessing particle safety, in terms of activation of innate/inflammatory reactions, and their possible exploitation for medical applications. In vitro assessment of nanoparticle-macrophage interaction allows for examining the response of primary human cells, but the conventional 2D cultures do not reproduce the three-dimensional spacing of a tissue and the interaction of macrophages with the extracellular tissue matrix, conditions that shape macrophage recognition capacity and reactivity. Here, we have compared traditional 2D cultures with cultures on a 3D collagen matrix for evaluating the capacity gold nanoparticles to induce monocyte activation and subsequent innate memory in human blood monocytes in comparison to bacterial LPS. Results show that monocytes react to stimuli almost in the same way in 2D and 3D cultures in terms of production of TNFα and IL-6, but that notable differences are found when IL-8 and IL-1Ra are examined, in particular in the recall/memory response of primed cells to a second stimulation, with the 3D cultures showing cell activation and memory effects of nanoparticles better. In addition, the response variations in monocytes/macrophages from different donors point towards a personalized assessment of the nanoparticle effects on macrophage activation.
Grants: European Commission 316383
European Commission 671881
European Commission 812661
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: Article ; recerca ; Versió publicada
Subject: Monocytes ; Macrophages ; Gold nanoparticles ; In vitro models ; Innate immunity ; Inflammation ; Innate memory ; 2D cultures ; 3D cultures
Published in: Nanomaterials, Vol. 11, issue 1 (Jan. 2021) , art. 207, ISSN 2079-4991

DOI: 10.3390/nano11010207
PMID: 33467414


13 p, 1.8 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles

 Record created 2021-05-28, last modified 2022-09-11



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