Diagnostic accuracy of the enhanced liver fibrosis (ELF®) score using HCV-Infected serum samples cryopreserved for up to 25 years
Puigvehí, Marc (Institut Hospital del Mar d'Investigacions Mèdiques)
Hernández, Juanjo (Laboratori de Referència de Catalunya (El Prat de Llobregat, Catalunya))
Broquetas, Teresa (Institut Hospital del Mar d'Investigacions Mèdiques)
Coll, Susanna (Institut Hospital del Mar d'Investigacions Mèdiques)
Garcia-Retortillo, M (Institut Hospital del Mar d'Investigacions Mèdiques)
Cañete Hidalgo, Nuria (Institut Hospital del Mar d'Investigacions Mèdiques)
Giménez, Maria Dolors (Institut Hospital del Mar d'Investigacions Mèdiques)
García, Mar (Hospital del Mar (Barcelona, Catalunya))
Bory, Felipe (Hospital del Mar (Barcelona, Catalunya))
Salvadó, Margarita (Laboratori de Referència de Catalunya (El Prat de Llobregat, Catalunya))
Solà, Ricard (Institut Hospital del Mar d'Investigacions Mèdiques)
Carrión Rodríguez, José Antonio (Institut Hospital del Mar d'Investigacions Mèdiques)
Universitat Autònoma de Barcelona

Date:	2016
Abstract:	Introduction & Aims Cryopreservation of serum samples is a standard procedure for biomedical research in tertiary centers. However, studies evaluating the long-term biological stability of direct liver fibrosis markers using cryopreserved samples are scarce. Methods We compared the stability of hyaluronic acid (HA), tissue inhibitor of metalloproteinases (TIMP-1) and amino-terminal propeptide of type III procollagen (PIIINP) in 225 frozen serum samples of HCV-infected patients with a paired liver biopsy for up to 25 years (1990-2014). Moreover, we assessed the diagnostic accuracy (AUROC) of the Enhanced Liver Fibrosis (ELF®) score to identify significant fibrosis (F2-4) and its predictive capacity to identify clinical events during follow-up. Results Seventy-six patients (39,8%) had mild fibrosis (F0-1) and 115 (60,2%) significant fibrosis (F2-4). HA, PIIINP and TIMP-1 values remained stable during the period from 1995 to 2014 while those of 1990-94 were slightly higher. We did not find significant differences in the median ELF® values during the 20-year period from 1995-2014 in patients with mild (from 8,4 to 8,7) and significant fibrosis (from 9,9 to 10,9) (p = ns between periods and fibrosis stages). The AUROCs of ELF® to identify significant fibrosis were high in all the periods (from 0,85 to 0,91). The ELF® score showed a good predictive capability to identify clinical events during follow-up. Conclusions The biological stability of direct serum markers (HA, PIIINP and TIMP-1) using HCV-infected samples cryopreserved for 20 years is good. Therefore, the diagnostic accuracy of the ELF® score to identify significant fibrosis and clinical events during follow-up is very high.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Adult ; Aged ; Biomarkers ; Cryopreservation ; Female ; Hepatitis C, Chronic ; Humans ; Hyaluronic Acid ; Liver Cirrhosis ; Male ; Middle Aged ; Peptide Fragments ; Procollagen ; Sensitivity and Specificity ; Tissue Inhibitor of Metalloproteinase-1 ; Young Adult
Published in:	PloS one, Vol. 11 Núm. 12 (december 2016) , p. e0164883, ISSN 1932-6203

DOI: 10.1371/journal.pone.0164883
PMID: 27984583

11 p, 1.1 MB

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