Electrochromogenic Detection of Live Bacteria Using Soluble and Insoluble Prussian Blue
Ferrer-Vilanova, Amparo (Institut de Microelectrònica de Barcelona)
Alonso, Yasmine (Universitat Autònoma de Barcelona. Departament de Química)
J. Ezenarro, Josune (Institut de Microelectrònica de Barcelona)
Santiago, Sara (Universitat Autònoma de Barcelona. Departament de Química)
Muñoz Berbel, Xavier (Institut de Microelectrònica de Barcelona)
Guirado López, Gonzalo (Universitat Autònoma de Barcelona. Departament de Química)

Fecha:	2021
Resumen:	Microbial detection is crucial for the control and prevention of infectious diseases, being one of the leading causes of mortality worldwide. Among the techniques developed for bacterial detection, those based on metabolic indicators are progressively gaining interest due to their simplicity, adaptability, and, most importantly, their capacity to differentiate between live and dead bacteria. Prussian blue (PB) may act as a metabolic indicator, being reduced by bacterial metabolism, producing a visible color change from blue to colorless. This molecule can be present in two main forms, namely, the soluble and the insoluble, having different properties and structures. In the current work, the bacterial-sensing capacity of soluble and insoluble PB will be tested and compared both in suspensions as PB-NPs and after deposition on transparent indium tin oxide-poly(ethylene terephthalate) (ITO-PET) electrodes. In the presence of live bacteria, PB-NPs are metabolized and completely reduced to the Prussian white state in less than 10 h for soluble and insoluble forms. However, when electrodeposited on ITO-PET substrates, less than 1 h of incubation with bacteria is required for both forms, although the soluble one presents faster metabolic reduction kinetics. This study paves the way to the use of Prussian blue as a metabolic indicator for the early detection of bacterial infection in fields like microbial diagnostics, surface sterilization, food and beverage contamination, and environmental pollution, among others.
Ayudas:	European Commission 720851 Ministerio de Economía y Competitividad CTQ2015-65439-R Agencia Estatal de Investigación PID2019-106171RB-I00 Agencia Estatal de Investigación RTC2019-007060-2
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Absorption ; Bacteria ; Color ; Electrodes ; Redox reactions
Publicado en:	ACS omega, Vol. 6, Issue 46 (November 2021) , p. 30989-30997, ISSN 2470-1343

DOI: 10.1021/acsomega.1c03434
PMID: 34841141

9 p, 5.5 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Grupo de Electroquímica, Fotoquímica y Reactividad Orgánica (GEFRO)
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