A pilot study of circulating levels of TGF-β1 and TGF-β2 as biomarkers of bone healing in patients with non-hypertrophic pseudoarthrosis of long bones
Chaverri, Daniel (Hospital ASEPEYO Sant Cugat)
Vivas, Daniel (Banc de Sang i Teixits)
Gallardo-Villares, Santiago (Hospital ASEPEYO Sant Cugat)
Granell-Escobar, Fernando (Hospital ASEPEYO Sant Cugat)
Pinto, Javier A. (Hospital ASEPEYO Sant Cugat)
Vives Armengol, Joaquim (Universitat Autònoma de Barcelona. Departament de Medicina)

Date:	2021
Abstract:	Pseudoarthrosis or non-union is a complication with an incidence of 5-10% of bone fractures, most frequently located in the diaphysis of long bones. The management of this complication is addressed by means of complex surgical procedures and is a concern for orthopaedic and trauma surgeons nowadays. The use of biomarkers for diagnosing patients at risk of non-union would help us to establish special measures for early corrective treatment. Prospective exploratory pilot study with a cohort of 20 patients diagnosed of non-hypertrophic pseudoarthrosis of long bones who were treated surgically with either autologous bone graft or a Tissue Engineering Product composed of bone marrow-derived Mesenchymal Stromal Cells. Patients were followed for 12 months and plasma blood samples were obtained to determine circulating levels of Transforming Growth Factor Beta 1 and Beta 2 (TGF-β1 and TGF-β2, respectively) at inclusion, and at 1 week, 2 weeks, and months 1, 2, 3, 6 and 12 after surgery. Radiological bone healing was evaluated by the Tomographic Union Score (TUS). Basal levels of TGF-β1 and TGF-β2 were determined in the twenty patients (26,702 ± 14,537 pg/mL and 307. 8 ± 83. 1 pg/mL, respectively). Three of them withdrew from the study, so complete follow-up was conducted on 17 patients (9 successfully healed vs. 8 that did not heal). Statistically significant differences between the bone healing group and the non-union group were found at month 12 for both TGF-β1 (p = 0. 005) and TGF-β2 (p = 0. 02). TGF-β1 and TGF-β2 are biomarkers that correlate with clinical evidence of bone regeneration and may be used to monitor patients, although early predictive value after intervention needs to be further studied in combination with other molecules.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Biomarker ; Blood sample ; Bone regeneration ; Pseudoarthrosis ; Non-union ; Multipotent Mesenchymal Stromal Cells ; Transforming Growth Factor-Beta 1 ; Transforming Growth Factor-Beta 2 ; Humans ; Clinical trial
Published in:	Bone Reports, Vol. 16 (december 2021) , ISSN 2352-1872

DOI: 10.1016/j.bonr.2021.101157
PMID: 34950754

8 p, 1.1 MB

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