Effects of intrauterine devices on proteins in the uterine lavage fluid of mares
Rivera del Álamo, Maria Montserrat (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Katila, Terttu (University of Helsinki. Department of Production Animal Medicine)
Palviainen, M. (University of Helsinki. Department of Production Animal Medicine)
Reilas, Tiina (Natural Resources Institute Finland)

Fecha:	2021
Resumen:	Intrauterine devices block luteolysis in cyclic mares, but the underlying mechanism is unknown. To clarify the mechanisms, the protein profile of the endometrial secretome was analyzed using two-dimensional difference gel electrophoresis (2D-DIGE). Twenty-seven mares were classified according to whether they were inseminated (AI) or had an intrauterine device (IUD), a water-filled plastic sphere, inserted into the uterus on Day 3 after ovulation. Uterine lavage fluids were collected on Day 15 from pregnant inseminated mares (AI-P; n = 8), non-pregnant inseminated mares (AI-N; n = 4), and mares with IUD (n = 15). The IUD group was further divided into prolonged (IUD-P; n = 7) and normal luteal phase (IUD-N; n = 8) groups on the basis of ultrasound examinations, serum levels of progesterone and PGFM on Days 14 and 15, and COX-2 results on Day 15. Four mares from each group were selected for the 2D-DIGE analyses. Ten proteins had significantly different abundance among the groups, nine of the proteins were identified. Malate dehydrogenase 1, increased sodium tolerance 1, aldehyde dehydrogenase 1A1, prostaglandin reductase 1, albumin and hemoglobin were highest in pregnant mares; T-complex protein 1 was highest in non-pregnant mares; and annexin A1 and 6-phosphogluconolactonase were highest in IUD mares. The results suggest that the mechanism behind the intrauterine devices is likely related to inflammation.
Nota:	Altres ajuts: acords transformatius de la UAB
Nota:	Altres ajuts: This work was supported by Finnish Veterinary Research, Agència de Gestió d'Ajuts Universitaris i de Recerca (AGAUR)
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Mare ; 2D-DIGE ; Endometrial proteins ; Intrauterine device ; Pregnancy
Publicado en:	Theriogenology, Vol. 165 (15 2021) , p. 1-9, ISSN 0093-691X

DOI: 10.1016/j.theriogenology.2021.02.001
PMID: 33601088

9 p, 1.5 MB

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