Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus
Soder Walz, Jesica M. (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Torrentó, Clara (Universitat de Barcelona. Departament de Mineralogia, Petrologia i Geologia Aplicada)
Algora, Camelia (Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Environmental Microbiology)
Wasmund, Kenneth (University of Vienna. Centre for Microbiology and Environmental Systems Science. Division of Microbial Ecology)
Cortés Garmendia, M. Pilar (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Soler, Albert (Universitat de Barcelona. Departament de Mineralogia, Petrologia i Geologia Aplicada)
Vicent i Huguet, Teresa (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Rosell, Mònica (Universitat de Barcelona. Departament de Mineralogia, Petrologia i Geologia Aplicada)
Marco Urrea, Ernest (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Fecha:	2022
Resumen:	Trichloromethane (TCM) is a pollutant frequently detected in contaminated aquifers, and only four bacterial strains are known to respire it. Here, we obtained a novel Dehalobacter strain capable of transforming TCM to dichloromethane, which was denominated Dehalobacter sp. strain 8M. Besides TCM, strain 8M also completely transformed 1,1,2-trichloroethane to vinyl chloride and 1,2-dichloroethane. Quantitative PCR analysis for the 16S rRNA genes confirmed growth of Dehalobacter with TCM and 1,1,2-trichloroethane as electron acceptors. Carbon and chlorine isotope fractionation during TCM transformation was studied in cultured cells and in enzymatic assays with cell suspensions and crude protein extracts. TCM transformation in the three studied systems resulted in small but significant carbon (εC = −2. 7 ± 0. 1‰ for respiring cells, −3. 1 ± 0. 1‰ for cell suspensions, and − 4. 1 ± 0. 5‰ for crude protein extracts) and chlorine (εCl = −0. 9 ± 0. 1‰, −1. 1 ± 0. 1‰, and − 1. 2 ± 0. 2‰, respectively) isotope fractionation. A characteristic and consistent dual Csingle bondCl isotope fractionation pattern was observed for the three systems (combined ΛC/Cl = 2. 8 ± 0. 3). This ΛC/Cl differed significantly from previously reported values for anaerobic dechlorination of TCM by the corrinoid cofactor vitamin B12 and other Dehalobacter strains. These findings widen our knowledge on the existence of different enzyme binding mechanisms underlying TCM-dechlorination within the genus Dehalobacter and demonstrates that dual isotope analysis could be a feasible tool to differentiate TCM degraders at field studies.
Ayudas:	Agencia Estatal de Investigación CTM2016-75587-C2-1-R Agencia Estatal de Investigación PID2019-103989RB-100 Agencia Estatal de Investigación CGL2017-87216-C4-1-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2017-SGR-14 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017-SGR-1733
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Trichloromethane ; 1,1,2-trichloroethane ; Dehalobacter ; Organohalide respiration ; Isotopic fractionation ; 2D-CSIA
Publicado en:	Science of the total environment, Vol. 813 (March 2022) , art. 152659, ISSN 1879-1026

DOI: 10.1016/j.scitotenv.2021.152659
PMID: 34954170

9 p, 1.0 MB

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