Encapsulation of combi-cleas of glycosidases in alginate beads and polyvinyl alcohol for wine aroma enhancement
Tavernini, Luigi (Pontificia Universidad Católica de Valparaíso. Escuela de Ingeniería Bioquímica)
Aburto, Carla (Pontificia Universidad Católica de Valparaíso. Escuela de Ingeniería Bioquímica)
Romero, Oscar (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Illanes, Andrés (Pontificia Universidad Católica de Valparaíso. Escuela de Ingeniería Bioquímica)
Wilson, Lorena (Pontificia Universidad Católica de Valparaíso. Escuela de Ingeniería Bioquímica)

Date:	2021
Abstract:	The aromatic expression of wines can be enhanced by the addition of specific glycosidases, although their poor stability remains a limitation. Coimmobilization of glycosidases as cross-linked enzyme aggregates (combi-CLEAs) offers a simple solution yielding highly stable biocatalysts. Nevertheless, the small particle size of combi-CLEAs hinders their recovery, preventing their industrial application. Encapsulation of combi-CLEAs of glycosidases in alginate beads and in polyvinyl alcohol is proposed as a solution. Combi-CLEAS of β-D-glucosidase and α-L-arabinofuranosidase were prepared and encapsulated. The effects of combi-CLEA loading and particle size on the expressed specific activity (IU/g) of the biocatalysts were evaluated. Best results were obtained with 2. 6 mm diameter polyvinyl alcohol particles at a loading of 60 mg/g, exhibiting activities of 1. 9 and 1. 0 IU/g for β-D-glucosidase and α-L-arabinofuranosidase, respectively. Afterwards, the stability of the biocatalysts was tested in white wine. All the encapsulated biocatalysts retained full activity after 140 incubation days, outperforming both free enzymes and nonencapsulated combi-CLEAs. Nevertheless, the alginate-encapsulated biocatalysts showed a brittle consistency, making recovery unfeasible. Conversely, the polyvinyl-encapsulated biocatalyst remained intact throughout the assay. The encapsulation of combi-CLEAs in polyvinyl alcohol proved to be a simple methodology that allows their recovery and reuse to harness their full catalytic potential.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Combi-CLEAs ; Glycosidases ; Wine aroma ; Alginate ; Polyvinyl alcohol
Published in:	Catalysts, Vol. 11, Issue 7 (July 2021) , art. 866, ISSN 2073-4344

DOI: 10.3390/catal11070866

9 p, 1.2 MB

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Articles > Research articles
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