Integrating molecularly imprinted polymer beads in graphite-epoxy electrodes for the voltammetric biosensing of histamine in wines
Herrera-Chacon, Anna (Universitat Autònoma de Barcelona. Departament de Química)
Dinç-Zor, Şule (Yildiz Technical University. Department of Chemistry)
Valle Zafra, Manuel del (Universitat Autònoma de Barcelona. Departament de Química)

Fecha:	2020
Descripción:	6 pàg.
Resumen:	This manuscript presents a voltammetric biosensing study with use of molecularly imprinted polymers to detect histamine in wine. Polymer beads were synthesized by standard precipitation polymerization method and implemented on the electrode surface via sol-gel immobilization. Scanning and confocal microscopy examinations permitted characterizing the material. Adsorptive stripping voltammetry in differential mode was the technique chosen for final application, selecting an enrichment time of 5 min. These conditions permitted a limit of detection of 0. 19 μg mL -1 (1. 0 μM), with a linear response range from 0. 5 to 6. 0 μg mL -1 (2. 71-32. 4 μM). The repeatability of the measurements was 4. 6% relative standard deviation (n = 12). Principal component analysis showed the ability of the prepared receptor for discriminating other biogenic amines and potential interfering species. A final application, illustrating the determination of histamine, was completed to show agreement of results between the fluorimetric reference method and the proposed electrochemical approach.
Ayudas:	Ministerio de Economía y Competitividad CTQ2016-80170-P
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió sotmesa a revisió
Materia:	Molecularly imprinted polymer ; Sensors ; Biogenic amines ; Histamine ; Voltammetry
Publicado en:	Talanta, Vol. 208 (Feb. 2020) , art. 120348, ISSN 1873-3573

DOI: 10.1016/j.talanta.2019.120348
PMID: 31816782

Preprint 22 p, 926.5 KB

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