Tetracycline and multidrug resistance in the oral microbiota : differences between healthy subjects and patients with periodontitis in Spain
Arredondo, Alexandre (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Blanc Pociello, Vanessa (Dentaid Research Center)
Mor, Carolina (Universitat Internacional de Catalunya. Facultat d'Odontologia)
Nart, José (Universitat Internacional de Catalunya. Facultat d'Odontologia)
León Berríos, Rubén (Dentaid Research Center. Department of Microbiolog)

Date:	2020
Abstract:	Introduction : Antibiotic resistance is widely found even among bacterial populations not having been exposed to selective pressure by antibiotics, such as tetracycline. In this study we analyzed the tetracycline-resistant subgingival microbiota of healthy subjects and of patients with periodontitis, comparing the prevalence of tet genes and their multidrug resistance profiles. Methods : Samples from 259 volunteers were analyzed, obtaining 813 tetracycline-resistant isolates. The prevalence of 12 antibiotic resistance genes was assessed, and multidrug profiles were built. Each isolate was identified by 16S rRNA sequencing. Differences in qualitative data and quantitative data were evaluated using the chi-square test and the Mann-Whitney-U test, respectively. Results : tet (M) was the most frequently detected tet gene (52. 03%). We observed significant differences between the prevalence of tet (M), tet (W), tet (O), tet (32) and tet (L) in both populations studied. Multidrug resistance was largely observed, with resistance to kanamycin being the most detected (83. 64%). There were significant differences between the populations in the prevalence of kanamycin, chloramphenicol, and cefotaxime resistance. Resistant isolates showed significantly different prevalence between the two studied groups. Conclusion : The high prevalence of multidrug resistance and tetracycline resistance genes found in the subgingival microbiota, highlights the importance of performing wider and more in-depth analysis of antibiotic resistance in the oral microbiota.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Antibiotic resistance ; Tetracycline ; Periodontitis ; Subgingival microbiota ; Multidrug resistance
Published in:	Journal of Oral Microbiology, Vol. 13, issue 1 (Nov. 2020) , art. 1847431, ISSN 2000-2297

DOI: 10.1080/20002297.2020.1847431
PMID: 33391624

9 p, 485.1 KB

The record appears in these collections:
Articles > Research articles
Articles > Published articles