Alteration of the Mitochondrial Effects of Ceria Nanoparticles by Gold : An Approach for the Mitochondrial Modulation of Cells Based on Nanomedicine
Gutiérrez-Carcedo, Patricia (Hospital Universitari Vall d'Hebron)
Navalón, Sergio (Universidad Politécnica de Madrid)
Simó Canonge, Rafael (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Setoain, Xavier (Hospital Clínic i Provincial de Barcelona)
Aparicio-Gómez, Carolina (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Abasolo, Ibane (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Victor, Victor Manuel (Universitat de València)
García, Hermenegildo (Universidad Politécnica de Madrid)
Herance, José Raul (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona

Fecha:	2020
Resumen:	Ceria nanoparticles are cell compatible antioxidants whose activity can be enhanced by gold deposition and by surface functionalization with positive triphenylphosphonium units to selectively target the mitochondria. The antioxidant properties of these nanoparticles can serve as the basis of a new strategy for the treatment of several disorders exhibiting oxidative stress, such as cancer, diabetes or Alzheimer's disease. However, all of these pathologies require a specific antioxidant according with their mechanism to remove oxidant species excess in cells and diminish their effect on mitochondrial function. The mechanism through which ceria nanoparticles neutralize oxidative stress and their effect on mitochondrial function have not been characterized yet. In the present study, the mitochondria antioxidant effect of ceria and ceria-supported gold nanoparticles, with or without triphenylphosphonium functionalization, was assessed in HeLa cells. The effect caused by ceria nanoparticles on mitochondria function in terms of mitochondrial membrane potential (∆Ψm), adenosine triphosphate (ATP) production, nuclear respiratory factor 1 (NRF1) and nuclear factor erythroid-2-like 1 (NFE2L1) was reversed by the presence of gold. Furthermore, this effect was enhanced when nanoparticles were functionalized with triphenylphosphonium. Our study illustrates how the mitochondrial antioxidant effect induced by ceria nanoparticles can be modulated by the presence of gold.
Ayudas:	Ministerio de Economía y Competitividad CP13/00252 Instituto de Salud Carlos III PI16/1083 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-13
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Mitochondrial function ; Ceria nanoparticles ; Gold-supported ceria nanoparticles ; Antioxidant ; Triphenylphosphonium gold-supported ceria nanoparticles
Publicado en:	Nanomaterials, Vol. 10 (april 2020) , ISSN 2079-4991

DOI: 10.3390/nano10040744
PMID: 32295053

16 p, 2.4 MB

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