Integrative omics analysis of the termite gut system adaptation to Miscanthus diet identifies lignocellulose degradation enzymes
Calusinska, Magdalena (Luxembourg Institute of Science and Technology. BioSystems and Bioprocessing Engineering)
Marynowska, Martyna (Université Libre de Bruxelles. Evolutionary Biology and Ecology)
Bertucci, Marie (Université Catholique de Louvain. Laboratory of Bioengineering, Earth and Life Institute)
Untereiner, Boris (Luxembourg Institute of Science and Technology. BioSystems and Bioprocessing Engineering)
Klimek, Dominika (Luxembourg Institute of Science and Technology. BioSystems and Bioprocessing Engineering)
Goux, Xavier (Luxembourg Institute of Science and Technology. BioSystems and Bioprocessing Engineering)
Sillam-Dussès, David (University Paris 13-Sorbonne Paris Cité. Laboratory of Experimental and Comparative Ethology)
Gawron, Piotr (University of Luxembourg. Luxembourg Centre for Systems Biomedicine)
Halder, Rashi (University of Luxembourg. Luxembourg Centre for Systems Biomedicine)
Wilmes, Paul (University of Luxembourg. Luxembourg Centre for Systems Biomedicine)
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Gerin, Patrick (Université Catholique de Louvain. Laboratory of Bioengineering, Earth and Life Institute)
Roisin, Yves (Université Libre de Bruxelles. Evolutionary Biology and Ecology)
Delfosse, Philippe (University of Luxembourg)

Fecha:	2020
Resumen:	Miscanthus sp. biomass could satisfy future biorefinery value chains. However, its use is largely untapped due to high recalcitrance. The termite and its gut microbiome are considered the most efficient lignocellulose degrading system in nature. Here, we investigate at holobiont level the dynamic adaptation of Cortaritermes sp. to imposed Miscanthus diet, with a long-term objective of overcoming lignocellulose recalcitrance. We use an integrative omics approach combined with enzymatic characterisation of carbohydrate active enzymes from termite gut Fibrobacteres and Spirochaetae. Modified gene expression profiles of gut bacteria suggest a shift towards utilisation of cellulose and arabinoxylan, two main components of Miscanthus lignocellulose. Low identity of reconstructed microbial genomes to closely related species supports the hypothesis of a strong phylogenetic relationship between host and its gut microbiome. This study provides a framework for better understanding the complex lignocellulose degradation by the higher termite gut system and paves a road towards its future bioprospecting. Through metagenomics and metatranscriptomics analyses, Calusinska et al. investigate the adaptation of the gut microbiome of the termite Cortaritermes sp. to a diet of Miscanthus grass. This work is a starting point for the identification of lignocellulose-degradation enzymes for potential biotechnology applications.
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Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Microbiome ; Applied microbiology
Publicado en:	Communications Biology, Vol. 3 (June 2020) , art. 275, ISSN 2399-3642

DOI: 10.1038/s42003-020-1004-3
PMID: 32483294

12 p, 2.0 MB

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