Cord Blood Platelet Rich Plasma Derivatives for Clinical Applications in Non-transfusion Medicine
Samarkanova, Dinara (Universitat Autònoma de Barcelona. Departament de Medicina)
Cox, Steven (Royal Free Hospital)
Hernandez, Diana (Royal Free Hospital)
Rodriguez, Luciano (Universitat Autònoma de Barcelona. Banc de Sang i Teixits)
Casaroli-Marano, Ricardo Pedro (Hospital Clínic i Provincial de Barcelona)
Madrigal, Alejandro (Royal Free Hospital)
Querol, Sergi (Hospital Universitari Vall d'Hebron)

Fecha:	2020
Resumen:	Cord blood platelet rich plasma (CB-PRP) derivatives have been investigated as potential therapeutic agents for the treatment of diverse conditions including ocular surface disease and skin ulcers. We have developed processes for the formulation of several CB-PRP preparations, which have different composition and attributes. Here we describe the molecular characteristics of these preparations and we make recommendations as to their most appropriate clinical application based on functional and immunomodulatory profiles. We show that incubation of adult peripheral blood mononuclear cells (PBMCs) with all three preparations dramatically reduced the production of INFγ and the expression of NKG2D and CD107a in NK, NKT, and T cells thus diminishing their activation, we propose that the likely mechanism is the high levels of soluble NKG2D ligands present in plasma. Of the three preparations we investigated, CB platelet lysate (PL) and platelet releaseate (PR) have higher concentrations of trophic and pro-angiogenic factors, CB platelet poor plasma (PPP) has the lowest concentration of all analytes measured. Based on these finding we propose that CB-PR is the most suitable raw material for skin wound patches, while CB-PL and PPP can be used to prepare eye drops for severe ocular surface pathologies and inflammatory conditions such as corneal ulcers or severe dry eye disease, respectively.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Cord blood ; Platelets rich plasma ; Plasma ; Platelets ; Ocular surface disease ; Skin ulcer ; Immunomodulation
Publicado en:	Frontiers in immunology, Vol. 11 (may 2020) , ISSN 1664-3224

DOI: 10.3389/fimmu.2020.00942
PMID: 32536916

14 p, 1.7 MB

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