Magnetite Nanoparticles Functionalized with RNases against Intracellular Infection of Pseudomonas aeruginosa
Rangel-Muñoz, Nathaly (Universidad de los Andes)
Suarez-Arnedo, Alejandra (Universidad de los Andes)
Anguita, Raúl (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Prats-Ejarque, Guillem (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Osma, Johann F. (Universidad de los Andes)
Muñoz-Camargo, Carolina (Universidad de los Andes)
Boix i Borràs, Esther (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Cruz, Juan C. (Universidad de los Andes)
Salazar, V.A (Universidad de los Andes)

Fecha:	2020
Resumen:	Current treatments against bacterial infections have severe limitations, mainly due to the emergence of resistance to conventional antibiotics. In the specific case of Pseudomonas aeruginosa strains, they have shown a number of resistance mechanisms to counter most antibiotics. Human secretory RNases from the RNase A superfamily are proteins involved in a wide variety of biological functions, including antimicrobial activity. The objective of this work was to explore the intracellular antimicrobial action of an RNase 3/1 hybrid protein that combines RNase 1 high catalytic and RNase 3 bactericidal activities. To achieve this, we immobilized the RNase 3/1 hybrid on Polyetheramine (PEA)-modified magnetite nanoparticles (MNPs). The obtained nanobioconjugates were tested in macrophage-derived THP-1 cells infected with Pseudomonas aeruginosa PAO1. The obtained results show high antimicrobial activity of the functionalized hybrid protein (MNP-RNase 3/1) against the intracellular growth of P. aeruginosa of the functionalized hybrid protein. Moreover, the immobilization of RNase 3/1 enhances its antimicrobial and cell-penetrating activities without generating any significant cell damage. Considering the observed antibacterial activity, the immobilization of the RNase A superfamily and derived proteins represents an innovative approach for the development of new strategies using nanoparticles to deliver antimicrobials that counteract P. aeruginosa intracellular infection.
Nota:	Altres ajuts: Fundació La Marató de TV3/20180310
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Pseudomonas aeruginosa ; Ribonucleases ; Magnetite nanoparticles ; Antimicrobials
Publicado en:	Pharmaceutics, Vol. 12, Num. 7 (July 2020) , art. 631, ISSN 1999-4923

DOI: 10.3390/pharmaceutics12070631
PMID: 32640506

25 p, 4.8 MB

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