Depletion of ATP Limits Membrane Excitability of Skeletal Muscle by Increasing Both ClC1-Open Probability and Membrane Conductance
Leermakers, Pieter Arnold (Aarhus University)
Dybdahl, Kamilla Løhde Tordrup (Aarhus University)
Husted, Kristian Søborg (Aarhus University)
Riisager, Anders (Aarhus University)
de Paoli, Frank Vincenzo (Aarhus University)
Pinós Figueras, Tomàs (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Vissing, John (Copenhagen University Hospital Rigshospitalet)
Krag, Thomas Oliver Brøgger (Copenhagen University Hospital Rigshospitalet)
Pedersen, Thomas Holm (Aarhus University)
Universitat Autònoma de Barcelona

Fecha:	2020
Resumen:	Activation of skeletal muscle contractions require that action potentials can be excited and propagated along the muscle fibers. Recent studies have revealed that muscle fiber excitability is regulated during repeated firing of action potentials by cellular signaling systems that control the function of ion channel that determine the resting membrane conductance (G ). In fast-twitch muscle, prolonged firing of action potentials triggers a marked increase in G , reducing muscle fiber excitability and causing action potential failure. Both ClC-1 and K ion channels contribute to this G rise, but the exact molecular regulation underlying their activation remains unclear. Studies in expression systems have revealed that ClC-1 is able to bind adenosine nucleotides, and that low adenosine nucleotide levels result in ClC-1 activation. In three series of experiments, this study aimed to explore whether ClC-1 is also regulated by adenosine nucleotides in native skeletal muscle fibers, and whether the adenosine nucleotide sensitivity of ClC-1 could explain the rise in G muscle fibers during prolonged action potential firing. First, whole cell patch clamping of mouse muscle fibers demonstrated that ClC-1 activation shifted in the hyperpolarized direction when clamping pipette solution contained 0 mM ATP compared with 5 mM ATP. Second, three-electrode G measurement during muscle fiber stimulation showed that glycolysis inhibition, with 2-deoxy-glucose or iodoacetate, resulted in an accelerated and rapid >400% G rise during short periods of repeated action potential firing in both fast-twitch and slow-twitch rat, and in human muscle fibers. Moreover, ClC-1 inhibition with 9-anthracenecarboxylic acid resulted in either an absence or blunted G rise during action potential firing in human muscle fibers. Third, G measurement during repeated action potential firing in muscle fibers from a murine McArdle disease model suggest that the rise in G was accelerated in a subset of fibers. Together, these results are compatible with ClC-1 function being regulated by the level of adenosine nucleotides in native tissue, and that the channel operates as a sensor of skeletal muscle metabolic state, limiting muscle excitability when energy status is low.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Skeletal muscle ; ATP ; Membrane excitability ; Fatigue ; Membrane conductance ; ClC-1 ; McArdle disease
Publicado en:	Frontiers in neurology, Vol. 11 (june 2020) , ISSN 1664-2295

DOI: 10.3389/fneur.2020.00541
PMID: 32655483

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