Effect of Topical Administration of Somatostatin on Retinal Inflammation and Neurodegeneration in an Experimental Model of Diabetes
Hernández, Cristina (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas)
Arroba, Ana I (Hospital Universitario Puerta del Mar (Cadis, Andalusia))
Bogdanov, Patricia (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas)
Ramos, Hugo (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas)
Simó-Servat, Olga (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas)
Simó Canonge, Rafael (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas)
Valverde, Angela M. (Instituto de Investigaciones Biomédicas "Alberto Sols")
Universitat Autònoma de Barcelona

Date:	2020
Abstract:	Somatostatin (SST) is a neuroprotective peptide but little is known regarding the potential role of its anti-inflammatory effects on retinal neuroprotection. In a previous study, we provided the first evidence that topical (eye drops) administration of SST prevents retinal neurodegeneration in streptozotocin (STZ)-induced diabetic rats. However, STZ by itself could cause neurotoxicity, thus acting as a confounding factor. The aims of the present study were: (1) to test the effect of topical administration of SST in the db/db mouse model, a spontaneous model of type 2 diabetes, thus avoiding the confounding effect of STZ on neurodegeneration; (2) to further explore the anti-inflammatory mechanisms of SST in glial cells. This task was performed by using mouse retinal explants and cell cultures. In summary, we confirm that SST topically administered was able to prevent retinal neurodysfunction and neurodegeneration in db/db mice. Furthermore, we found that SST prevented the activation of the classical M1 response of Bv. 2 microglial cells upon Lipopolysaccharide (LPS) stimulation as a potent pro-inflammatory trigger. The anti-inflammatory effect of SST in Bv. 2 cells was also observed in response to hypoxia. In conclusion, we provide evidence that the neuroprotective effect of SST in diabetic retinas can be largely attributed to anti-inflammatory mechanisms.
Grants:	European Commission. Horizon 2020 278040 Ministerio de Economía y Competitividad BES-2017-08169
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Somatostatin ; Retinal neurodegeneration ; Retinal inflammation ; Diabetic retinopathy ; Microglia ; Db/db mice
Published in:	Journal of clinical medicine, Vol. 9 (august 2020) , ISSN 2077-0383

DOI: 10.3390/jcm9082579
PMID: 32784955

18 p, 4.6 MB

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