Nanoplasmonic Paper-Based Platform for General Screening of Biomacromolecules
Pujol Vila, Ferran (Institut de Microelectrònica de Barcelona)
Aveling Jenkins, Andrew Tobias (University of Bath. Department of Chemistry)
Muñoz Berbel, Xavier (Institut de Microelectrònica de Barcelona)
Mas Gordi, Jordi (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Fecha:	2020
Resumen:	Hygiene assessment in industrial and clinical environments is crucial in the prevention of health risks. Current technologies for routine cleanliness evaluation rely on the detection of specific biomolecules, thus requiring more than one test for broad-range screening. Herein, the modulation of the catalytic activity of gold nanoparticles (AuNPs) by biomacromolecules was employed to develop a nanoplasmonic platform for general hygiene screening. AuNPs were immobilized on cellulose paper by simple adsorption. When ferricyanide was dispensed onto the paper, the AuNPs catalysed the ferricyanide's dissociation, releasing free cyanide ions that dissolved them. The AuNP dissolution produced an intense colour shift detectable with the naked eye. When biomacromolecules (e. g. , proteins and polysaccharides) were present, they spontaneously attached to AuNPs, forming a biomolecular corona (biocorona), reducing their catalytic activity until complete suppression when the NPs were fully covered by molecules. The concentration-dependent decrease in the catalytic activity was here used to quantify biomacromolecules and complex samples such as milk, eggs, soy sauce and yeast extract (in 20 min), with detection limits comparable to those of standard methods, i. e. , 0. 25 µg mL -1 for albumin. This nano-enabled technology may be applied as a broad-range (unspecific) alert system for inexpensive cleanliness evaluation, with potential applications in sensitive sectors including productive industries and hospitals.
Ayudas:	Ministerio de Economía y Competitividad CTQ2014-54553-C3-2-R Ministerio de Economía y Competitividad RTC-2016-5766-2
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Nanoplasmonic platform ; Gold nanoparticles ; Biomolecular coronas ; Nanocatalytic activity ; Hygiene screening
Publicado en:	Nanomaterials, Vol. 10, Issue 12 (November 2020) , art. 2335, ISSN 2079-4991

DOI: 10.3390/nano10122335
PMID: 33255587

13 p, 2.7 MB

El registro aparece en las colecciones:
Artículos > Artículos de investigación
Artículos > Artículos publicados