The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes
Nieto-Fontarigo, Juan José (Universidade de Santiago de Compostela)
Salgado, F. J. (Universidade de Santiago de Compostela)
San-José, M. E. (Complejo Hospitalario Universitario de Santiago de Compostela)
Cruz Carmona, María Jesús (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Casas-Fernández, A. (Complejo Hospitalario Universitario de Santiago de Compostela)
Gómez-Conde, M. J. (Complejo Hospitalario Universitario de Santiago de Compostela)
Valdés-Cuadrado, L. (Complejo Hospitalario Universitario de Santiago de Compostela)
García-González, M. Á. (Instituto de Investigación Sanitaria de Santiago (IDIS))
Arias, Pilar (Universidade de Santiago de Compostela)
Nogueira, Montserrat (Universidade de Santiago de Compostela)
González-Barcala, Francisco-Javier (Complejo Hospitalario Universitario de Santiago de Compostela)
Universitat Autònoma de Barcelona

Date:	2018
Abstract:	LPS-ligation to CD14/TLR-4 on monocytes/macrophages triggers the production of IL-12-family cytokines. IL12/18 promote TH-differentiation, counteracting the TH-driven asthma. Therefore, CD14 modulation could alter the TH-differentiation and should be taken into account when studying asthma. To analyse the alteration in CD14 levels and its association with CD14 (−159 C/T) SNP (rs2569190) in Caucasian adults with stable allergic asthma, we performed a cross-sectional study (277 healthy subjects vs. 277 patients) where clinical parameters, CD14 values and the CD14 (−159 C/T) SNP were studied. Apart from typical biomarkers, we found an increment of neuron-specific enolase (NSE) in allergic asthma, probably linked to monocyte activity. Indeed, we evidenced increased monocyte numbers, but lower CD14 expression and normalised sCD14 values in patients. Moreover, we noticed an association of the T allele (P = 0. 0162) and TT genotype (P = 0. 0196) of the CD14 SNP with a decreased risk of allergic asthma and augmented sCD14 levels. In conclusion, monocyte CD14 expression and normalized sCD14 values were reduced in stable state asthmatics, and this could be related to the presence of an expanded CD14 low monocyte subset. This study also demonstrates that the CD14 (−159 C/T) polymorphism is a risk factor for moderate-severe allergic asthma in adult Caucasians.
Grants:	Instituto de Salud Carlos III PI13/02046
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Scientific reports, Vol. 8 (march 2018) , ISSN 2045-2322

DOI: 10.1038/s41598-018-20483-1
PMID: 29515128

10 p, 1.6 MB

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