Skeletal muscle metabolomics and blood biochemistry analysis reveal metabolic changes associated with dietary amino acid supplementation in dairy calves
Yu, Kuai (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Matzapetakis, Manolis (Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier)
Valent, Daniel (Universitat Autònoma de Barcelona. Servei de Bioquímica Clínica Veterinària)
Saco Rodriguez, Yolanda (Universitat Autònoma de Barcelona. Servei de Bioquímica Clínica Veterinària)
De Almeida, André M. (Universidade de Lisboa. Instituto Superior de Agronomia)
Terré, Marta (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Bassols Teixidó, Anna Maria (Universitat Autònoma de Barcelona. Servei de Bioquímica Clínica Veterinària)

Fecha:	2018
Resumen:	The effects of different amino acid (AA) supplementations of milk protein-based milk replacers in pre-ruminant calves from 3 days to 7 weeks of age were studied. Animals were divided into 4 groups: Ctrl) Control group fed with milk protein-based milk replacer without supplementation; GP) supplementation with 0. 1% glycine and 0. 3% proline; FY) supplementation with 0. 2% phenylalanine and 0. 2% tyrosine; MKT) supplementation with 0. 62% lysine, 0. 22% methionine and 0. 61% threonine. For statistical analysis, t -test was used to compare AA-supplemented animals to the Ctrl group. At week 7, body weight and average daily gain (ADG) were measured and blood samples and skeletal muscle biopsies were taken. Blood biochemistry analytes related to energy metabolism were determined and it was shown that MKT group had higher serum creatinine and higher plasma concentration of three supplemented AAs as well as arginine compared with the Ctrl group. GP group had similar glycine/proline plasma concentration compared with the other groups while in FY group only plasma phenylalanine concentration was higher compared with Control. Although the AA supplementations in the GP and FY groups did not affect average daily gain and metabolic health profile from serum, the metabolome analysis from skeletal muscle biopsy revealed several differences between the GP-FY groups and the Ctrl-MKT groups, suggesting a metabolic adaptation especially in GP and FY groups.
Ayudas:	Ministerio de Economía y Competitividad AGL2015-68463-C2-2-P
Nota:	Altres ajuts: European Cooperation in Science and Technology COST-STSM-FA1308-36644
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Scientific reports, Vol. 8 (september 2018) , ISSN 2045-2322

DOI: 10.1038/s41598-018-32241-4
PMID: 30218007

12 p, 1.4 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
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