Effects of diesel exhaust particle exposure on a murine model of asthma due to soybean
Álvarez-Simón, Daniel (Universitat Autònoma de Barcelona. Departament de Medicina)
Gómez-Ollés, Susana (Centro de Investigación Biomédica en Red de Enfermedades Respiratorias)
de Homdedeu, Miquel (Hospital Universitari Vall d'Hebron)
Untoria, María-Dolores (Centro de Investigación Biomédica en Red de Enfermedades Respiratorias)
Cruz Carmona, María Jesús (Centro de Investigación Biomédica en Red de Enfermedades Respiratorias)
Muñoz Gall, Xavier (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Fecha:	2017
Resumen:	Exposure to soybean allergens has been linked to asthma outbreaks. Exposure to diesel exhaust particles (DEP) has been associated with an increase in the risk of asthma and asthma exacerbation; however, in both cases the underlying mechanisms remain poorly understood, as does the possible interaction between the two entities. To investigate how the combination of soybean allergens and DEP can affect the induction or exacerbation of asthma in a murine model. BALB/c mice received intranasal instillations of saline, 3 or 5 mg protein/ml soybean hull extract (SHE), or a combination of one of these three solutions with DEP. Airway hyperresponsiveness (AHR), pulmonary inflammation in bronchoalveolar lavage, total serum immunoglobulin E and histological studies were assessed. A 5 mg protein/ml SHE solution was able by itself to enhance AHR (p = 0. 0033), increase eosinophilic inflammation (p = 0. 0003), increase levels of IL-4, IL-5, IL-13, IL-17A, IL-17F and CCL20, and reduce levels of IFN-γ. The combination of 5 mg protein/ml SHE with DEP also produced an increase in AHR and eosinophilic inflammation, but presented a slightly different cytokine profile with higher levels of Th17-related cytokines. However, while the 3 mg protein/ml SHE solution did not induce asthma, co-exposure with DEP resulted in a markedly enhanced AHR (p = 0. 002) and eosinophilic inflammation (p = 0. 004), with increased levels of IL-5, IL-17F and CCL20 and decreased levels of IFN-γ. The combination of soybean allergens and DEP is capable of triggering an asthmatic response through a Th17-related mechanism when the soybean allergen concentration is too low to promote a response by itself. DEP monitoring may be a useful addition to allergen monitoring in order to prevent new asthma outbreaks.
Ayudas:	Ministerio de Economía y Competitividad CP12/03101
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	PloS one, Vol. 12 (june 2017) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0179569
PMID: 28628664

14 p, 6.3 MB

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